修饰抗体 Products
组蛋白赖氨酸三甲基化 (tri-methylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-tri-methyl-Histone H4 (Lys20) mouse mAb

Cat#:

PTM-665

Ab Type:

M

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by tri-methylated histone H4 (Lys20) peptide affinity chromatography. It specifically recognizes histone H4 with tri-methylation at Lys20.

 

Source/Purification

This product is produced by immunizing mice with a synthetic tri-methyl peptide corresponding to residues surrounding Lys20 of human histone H4. Antibodies are purified by protein G-conjugated agarose followed by tri-methylated histone H4 (Lys20) peptide affinity chromatography.  


Specificity

H4

Recommended Applications

ELISA, WB; Not tested in other applications  

*WB=Western blotting;

  

Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications.  

Anti-tri-methyl-Histone H4 (Lys20) mouse mAb

A. Tri-methyl-Histone H4 (Lys20) mouse mAb specificity was determined by peptide ELISA. The graph depicts the binding of the antibody to pre-coated tri-methyl-histone H4 (Lys20) peptide in the presence of increasing concentrations of various competitor peptides. As shown, only the tri-methyl-histone H4 (Lys20) peptide competed away binding of the antibody.  


B. Western blotting analysis on 30 μg of crude proteins from HeLa, JurKat and BRL whole cell lysates using tri-methyl-histone H4 (Lys20) mouse mAb (1:2000).  

 

Scientific Description

The ε-amino lysine methylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine methylation in multiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation in core histones is a major determinant for the formation of active and inactive regions of the genome and therefore plays vital roles in multiple cellular events. In most species, lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79), and H4 (Lys20) and has been implicated in both transcriptional activation and silencing. Methylation in histones modulated by specific histone methylases (HMTs) and histone demethylases (HDMs). The level of methyllysineis found impaired in the pathologies of cancers and other diseases, therefore, enzymes regulating histone lysine methylation have become promising targets for anti-cancer drugs.  


Storage & Stability

Store product at -20oC.



ELISA, WB; Not tested in other applications  

*WB=Western blotting;


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