修饰抗体 Products
组蛋白磷酸化(phosphorylation)修饰抗体
杭州景杰生物科技有限公司
Anti-phospho-Histone H2A.X (Ser139) mouse mAb

Cat#:

PTM-736

Ab Type:

M

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

2880.00

Price: (20 μl)

700.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by phosphorylated histone H2A.x (Ser139) peptide affinity chromatography. It specifically recognizes histone H2A.x with serine phosphorylation at Ser139.


Source/Purification

This product is produced by immunizing mice with a synthetic phosphoryl peptide corresponding to residues surrounding Ser139 of human histone H2A.x. Antibodies are purified by protein G-conjugated agarose followed by phosphorylated histone H2A.x (Ser139) peptide affinity chromatography.


Specificity

H2A.X

Recommended Applications

ELISA,WB, IP; Not tested in other applications   

*WB=Western blotting IP=Immunoprecipitation


Recommended antibody dilution

WB: 1:2000 IP: 1:25~1:200

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

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Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation and newly discovered succinylation, crotonylation and 2-hydroxyisobutyrylation, etc. These modifications have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. Histone H2A.x is a ubiquitously expressed variant of histone H2A and phosphorylation of histone H2A.x at Ser139 (termed γ-H2AX) is located in a unique C-terminal region and regulated by various kinases, including the ataxia telangiectasia-mutated (ATM) protein, the AT and Rad3-related protein, and DNA-dependent protein kinase, all of which were reported to be involved in the responses of mammalian cells to DNA double-strand breaks.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



ELISA,WB, IP; Not tested in other applications   

*WB=Western blotting IP=Immunoprecipitation


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