修饰抗体 Products
组蛋白磷酸化(phosphorylation)修饰抗体
杭州景杰生物科技有限公司
Anti-phospho-Histone H1.B (Thr146) rabbit pAb

Cat#:

PTM-722

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

2880.00

Price: (20 μl)

700.00


Product Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation and newly discovered succinylation, crotonylation and 2-hydroxyisobutyrylation, etc. These modifications have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. Histone H2A phosphorylation is specifically phosphorylated during both mitosis and meiosis that is believed to be mainly involved in transcriptional activation requiring chromatin fiber decondensation, and chromosome compaction during cell division. Histone H2Aphosphorylation may initiate at different phases of the cell division in different organisms, but metaphase chromosomes are always found to be heavily phosphorylated.

 

Source/Purification

This product is produced by immunizing rabbits with a synthetic phosphoryl peptide corresponding to residues surrounding Thr146 of human histone H1.B. Antibodies are purified by protein A-conjugated agarose followed by phosphorylated histone H1.B (Thr146) peptide affinity chromatography.  


Specificity

H1.B

Recommended Applications

ELISA,WB, IP; Not tested in other applications   

*WB=Western blotting IP=Immunoprecipitation


Recommended antibody dilution

WB: 1:2000 IP: 1:25~1:500

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

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Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation and newly discovered succinylation, crotonylation and 2-hydroxyisobutyrylation, etc. These modifications have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. Histone H2A phosphorylation is specifically phosphorylated during both mitosis and meiosis that is believed to be mainly involved in transcriptional activation requiring chromatin fiber decondensation, and chromosome compaction during cell division. Histone H2Aphosphorylation may initiate at different phases of the cell division in different organisms, but metaphase chromosomes are always found to be heavily phosphorylated.

 

Storage & Stability

Store product at -20 oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.  

 

**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.  


ELISA,WB, IP; Not tested in other applications   

*WB=Western blotting IP=Immunoprecipitation


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