修饰抗体 Products
组蛋白磷酸化(phosphorylation)修饰抗体
杭州景杰生物科技有限公司
Anti-tri-methyl and phospho-Histone H3 (Lys9/Ser10) rabbit pAb

Cat#:

PTM-723

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

2880.00

Price: (20 μl)

700.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by tri-methyl(Lys9)/phospho(ser10) histone H3 peptide affinity chromatography. It specifically recognizes histone H3 only when it tri-methylated at Lys9 and phosphorylated at Ser10.  


Source/Purification

This product is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Lys9 and ser10 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by tri-methylated (lys 9) and phosphorylated (Ser10) histone H3 peptide affinity chromatography.  


Specificity

H3

Recommended Applications

ELISA, WB; Not tested in other applications  

*WB=Western blotting  


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user.  

Anti-tri-methyl and phospho-Histone H3 (Lys9/Ser10) rabbit pAb

A. Dot blotting analysis on indicated amount of tri-methyl(Lys9)/phospho(Ser10) H3 peptide (lane 1), phosphorylated H3 peptide at Thr3 (lane 2), crotonylated H3 peptide at Lys4 (lane 3), mono-methylated H3 peptide at Lys4 (lane 4), di-methylated H3 peptide at Lys4 (lane 5), tri-methylated H3 peptide at Lys4 (lane 6), phosphorylated H3 peptide at Thr6 (lane 7), acetylated H3 peptide at Lys9 (lane 8), butyrylated H3 peptide at Lys9 (lane 9), 2ohibutyrylated H3 peptide at Lys9 (lane 10), 3ohbutyrylated H3 peptide at Lys9 (lane 11), mono-methylated H3 peptide at Lys9 (lane 12), di-methylated H3 peptide at Lys9 (lane 13), tri-methylated H3 peptide at Lys9 (lane 14), phosphorylated H3 peptide at Ser10 (lane 15), crotonylated H3 peptide at Lys14 (lane 16), mono-methylated H3 peptide at Lys14 (lane 17), di-methylated H3 peptide at Lys14 (lane 18), and unmodified H3 peptide at Lys9/Ser10 (lane 19) using anti-tri-methyl (Lys9)/ phosphor(ser10) histone H3  rabbit pAb.

 Anti-tri-methyl and phospho-Histone H3 (Lys9/Ser10) rabbit pAb
 

B. Western blotting analysis on 30 μg of crude proteins from human JK whole cell lysates with (Lane 2) or without (Lane 1) treatment of etopside (100 μg/ml, 24hr) and calyculin A ( CA, 100 nM, 1hr) using anti-tri-methyl (Lys9)/ phosphor(ser10) histone H3 rabbit pAb (1:2000).


C. Western blotting analysis on 30 μg of crude proteins from mouse C2C12 whole cell lysates with (Lane 2) or without (Lane 1) treatment of nocodazole (100 ng/ml, 18hr) and calyculin A ( CA, 100 nM, 1hr) using anti-tri-methyl (Lys9)/ phosphor(ser10) histone H3 rabbit pAb (1:2000).


D. Western blotting analysis on 30 μg of crude proteins from mouse spleen and liver whole tissue lysates using anti-tri-methyl (Lys9)/ phosphor(ser10) histone H3 rabbit pAb (1:500).  

 

Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation and newly discovered succinylation, crotonylation and 2-hydroxyisobutyrylation, etc. These modifications have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. Histone H3 phosphorylation is specifically phosphorylated during both mitosis and meiosis that is believed to be mainly involved in transcriptional activation requiring chromatin fiber decondensation, and chromosome compaction during cell division. Histone H3 phosphorylation may initiate at different phases of the cell division in different organisms, but metaphase chromosomes are always found to be heavily phosphorylated.

 

Storage & Stability

Store product at -20oC.



ELISA, WB; Not tested in other applications  

*WB=Western blotting  


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