修饰抗体 Products
组蛋白磷酸化(phosphorylation)修饰抗体
杭州景杰生物科技有限公司
Phospho-Histone H3 (Thr32) rabbit pAb

Cat#:

PTM-720

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

2880.00

Price: (20 μl)

700.00


Product Description
Source/Purification

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by phosphorylated histone H3 (Thr32) peptide affinity chromatography. It specifically recognizes histone H3 with threonine phosphorylation at Thr32.

 

Specificity

H3

Recommended Applications

ELISA, Dot blot, Western blot.

Recommended antibody dilution: WB: 1:2000

 

Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

捕获28.JPG

捕获29.JPG

A.Dot blotting analysis on indicated amount of peptides using histone H3T120ph rabbit pAb. The list of peptides is included in the above table.

B.Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (right) or without (left) treatment of nocodazole (100 ng/ml, 18hr) and calyculin A (CA,100 nM, 1hr) using phospho-histone H3 (Thr32) rabbit pAb (1: 2000).

C.Western blotting analysis on 30 μg of crude proteins from C2C12 whole cell lysates with (right) or without (left) treatment of nocodazole (100 ng/ml, 18hr) and calyculin A (CA,100 nM, 1hr) using phospho-histone H3 (Thr32) rabbit pAb (1: 2000).


Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation and newly discovered succinylation, crotonylation and 2-hydroxyisobutyrylation, etc. These modifications have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. Histone H2A phosphorylation is specifically phosphorylated during both mitosis and meiosis that is believed to be mainly involved in transcriptional activation requiring chromatin fiber decondensation, and chromosome compaction during cell division. Histone H2Aphosphorylation may initiate at different phases of the cell division in different organisms, but metaphase chromosomes are always found to be heavily phosphorylated.

 

Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.

 


ELISA, Dot blot, Western blot.

Recommended antibody dilution: WB: 1:2000


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