修饰抗体 Products
组蛋白磷酸化(phosphorylation)修饰抗体
杭州景杰生物科技有限公司
Anti-phospho-Histone H3 (Thr45) rabbit pAb

Cat#:

PTM-724

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

2880.00

Price: (20 μl)

700.00


Product Description
Source/Purification

This product is produced by immunizing rabbits with a synthetic phosphoryl peptide corresponding to residues surrounding Thr45 of human histone H3. Antibodies are purified by protein A-conjugated agarosefollowed by phosphorylated histone H3 (Thr45) peptide affinity chromatography.


Specificity

H3

Recommended Applications
ELISA, Dot blot, Western blot.  
Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

捕获18.JPG

捕获19.JPG

A.Dot blotting analysis on indicated amount of peptides using histone H3T45ph rabbit pAb. The list of peptides is included in the table on the right.


B.Western blotting analysis on 30 μg of crude proteins from JK whole cell lysates with (right) or without (left) treatment of calyculin A (100 nM, 1hr) and 12-O-tetradecanoylphorbol- 13-acetate(TPA) (200nM, 30 min) (Figure B) and 30 μg of crude proteins from HeLa whole cell lysates with (right) or without (left) treatment of nocodazole(100 ng/ml, 18hr) and calyculin A (100 nM, 1hr) (Figure C) using anti-H3T45ph rabbit pAb (1:2000).  


Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation and newly discovered succinylation, crotonylation and 2-hydroxyisobutyrylation, etc. These modifications have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. Histone H3 phosphorylation is specifically phosphorylated during both mitosis and meiosis that is believed to be mainly involved in transcriptional activation requiring chromatin fiber de-condensation, and chromosome compaction during cell division. Histone H3 phosphorylation may initiate at different phases of the cell division in different organisms, but metaphase chromosomes are always found to be heavily phosphorylated.


Storage & Stability

The antibody is delivered in powder form. Before use, please read carefully the Reconstitution and Storage Instructions. After dissolved with the antibody reconstitution buffer, please store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt.



ELISA, Dot blot, Western blot.  


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