修饰抗体 Products
组蛋白磷酸化(phosphorylation)修饰抗体
杭州景杰生物科技有限公司
Anti-phospho-Histone H3 (Ser10) mouse mAb

Cat#:

PTM-732

Ab Type:

M

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

2880.00

Price: (20 μl)

700.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by phosphorylated histone H3 (Ser10) peptide affinity chromatography. It specifically recognizes histone H3 with serine phosphorylation at Ser10.


Source/Purification

This product is produced by immunizing mice with a synthetic phospho peptide corresponding to residues surrounding Ser10 of human histone H3. Antibodies are purified by protein G-conjugated agarose followed by phosphorylated histone H3 (Ser10) peptide affinity chromatography.


Specificity

H3

Recommended Applications

ELISA, WB, IP; Not tested in other applications

*WB=Western blotting; IP= Immunoprecipation


Recommended antibody dilution

WB: 1:2000 IP: 1:100

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

 Anti-phospho-Histone H3 (Ser10) mouse mAb   

A. Dot  blotting analysis on indicated amount of phosphorylated H3 peptide at Ser10  (lane 1), phosphorylated H3 peptide at Ser28 (lane 2), phosphorylated H3  peptide at Thr3 (lane 3), phosphorylated H3 peptide at Thr11 (lane 4) and unmodified  H3 peptide at Ser10 (lane 5) using phospho-histone H3 (Ser10) mouse mAb.


B. Western blotting analysis on 30 μg of crude proteins  from HeLa whole cell lysates with (lane 2) or without (lane 1) treatment of nocodazole  (100 ng/ml, 18hr) and calyculin A ( CA, 100 nM, 1hr) using phospho -histone H3  (Ser10) mouse mAb (1:2000).


Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation and newly discovered succinylation, crotonylation and 2-hydroxyisobutyrylation, etc. These modifications have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. Histone H3 phosphorylation is specifically phosphorylated during both mitosis and meiosis that is believed to be mainly involved in transcriptional activation requiring chromatin fiber decondensation, and chromosome compaction during cell division. Histone H3 phosphorylation may initiate at different phases of the cell division in different organisms, but metaphase chromosomes are always found to be heavily phosphorylated.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



ELISA, WB, IP; Not tested in other applications

*WB=Western blotting; IP= Immunoprecipation


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