修饰抗体 Products
组蛋白赖氨酸丙酰化 (propionylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-propionyl-Histone H2A (Lys125) rabbit pAb

Cat#:PTM-217Ab Type:P

Ab Size:100Applications:WB

Species:H M RPrice:¥ 3780.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by propionylated Histone H2A (Lys125) peptide affinity chromatography. It specifically recognizes Histone H2A with propionylation at Lys125.

  

Source/Purification

This product is produced by immunizing rabbits with a synthetic propionyl peptide corresponding to residues surrounding Lys125 of human histone H2A. Antibodies are purified by protein A-conjugated agarose followed by propionylated histone H2A (Lys125) peptide affinity chromatography.  


Specificity

H2A

Recommended Applications

ELISA, WB; Not tested in other applications  

*WB=Western blotting;  


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

捕获21.JPG

捕获20.JPG

A. Dot blotting analysis on indicated amount of propionylated H2A peptide at Lys125 (lane 1), acetylated H2A peptide at Lys118 (lane 2), crotonylated H2A peptide at Lys118 (lane 3), 2ohibutylated H2A peptide at Lys118 (lane 4), 3ohbutyrylated H2A peptide at Lys118 (lane 5), crotonylated H2A peptide at Lys119 (lane 6), ubiquitinated H2A peptide at Lys119 (lane 7), acetylated H2A peptide at Lys125 (lane 8), and unmodified H2A peptide at Lys125 (lane 9) using propionyl-Histone H2A (Lys125) rabbit pAb.  


B. Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (Fig B. lane 2) or without (Fig B. lane 1) sodium butyrate treatment (30 mM, 4 hours) using propionyl-Histone H2A (Lys125) rabbit pAb (1:1000).  


C. Western blotting analysis on 30 μg of crude proteins from C2C12 whole cell lysates with (Fig C. lane 2) or without (Fig C. lane 1) sodium butyrate treatment (30 mM, 4 hours) using propionyl-Histone H2A (Lys125) rabbit pAb (1:1000).  


Scientific Description

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Lysine propionylation (Kprop) is structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histone H3 at Lys12, propionylation of histone H2A at Lys125 may play a vital role in the epigenetic modulation, including chromatin remodeling and transcriptional regulation.

 

Storage & Stability

Store product at -20oC.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.  



1. Zhang K, Chen Y, Zhang Z, et al. J Proteome Res. 2009, 8(2):900-906  

2. Cheng Z, Tang Y, Chen Y,et al. Mol Cell Proteomics. 2009, 8(1):45-52.  

3. Chen Y, Sprung R, Tang Y, et al. Mol Cell Proteomics. 2007, 6(5):812-819.  


ELISA, WB; Not tested in other applications  

*WB=Western blotting;  


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