修饰抗体 Products
组蛋白赖氨酸丙酰化 (propionylation) 修饰抗体
杭州景杰生物科技有限公司
Propionyl-Histone H3 (Lys14) mouse mAb

Cat#:PTM-215Ab Type:M

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description

This product is produced by immunizing mice with a synthetic propionyl peptide corresponding to residues surrounding Lys14 of human histone H3. Antibodies are purified by protein G-conjugated agarose followed by propionylated histone H3 (Lys14) peptide affinity chromatography.


Source/Purification

This product is produced by immunizing mice with a synthetic propionyl peptide corresponding to residues surrounding Lys14 of human histone H3. Antibodies are purified by protein G-conjugated agarose followed by propionylated histone H3 (Lys14) peptide affinity chromatography.


Specificity

H3

Recommended Applications

ELISA, WB; Not tested in other applications

 *WB=Western blotting


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking.

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user.

Propionyl-Histone H3 (Lys14) mouse mAb


A. Dot blotting analysis on indicated amount of propionylated H3 peptide at Lys14 (lane 1), acetylated H3 peptide at Lys9 (lane 2), butyrylated H3 peptide at Lys9 (lane 3), crotonylated H3 peptide at Lys9 (lane 4), 2ohibutyrylated H3 peptide at Lys9 (lane 5), 3ohbutyrylated H3 peptide at Lys9 (lane 6), acetylated H3 peptide at Lys14 (lane 7), butyrylated H3 peptide at Lys14 (lane 8), succinylated H3 peptide at Lys14 (lane 9), crotonylated H3 peptide at Lys14 (lane 10), 2ohibutyrylated H3 peptide at Lys14 (lane 11), 3ohbutyrylated H3 peptide at Lys14 (lane 12), mono-methylated H3 peptide at Lys14 (lane 13), di-methylated H3 peptide at Lys14 (lane 14), acetylated H3 peptide at Lys18 (lane 15), propionylated H3 peptide at Lys18 (lane 16), butyrylated H3 peptide at Lys18 (lane 17), crotonylated H3 peptide at Lys18 (lane 18), 2ohibutyrylated H3 peptide at Lys18 (lane 19), 3ohbutyrylated H3 peptide at Lys18 (lane 20), and unmodified H3 peptide at Lys14 (lane 21) using propionyl-Histone H3 (Lys14) mouse mAb.


B. Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (right) or without (left) sodium propionate treatment (10 mM, 4 hours) using propionyl-Histone H3 (Lys14) mouse mAb (1:2000).


Scientific Description

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Lysine propionylation (Kprop) is structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histione H3 at Lys12, propionylation of histone H3 at Lys14 may play a vital role in the epigenetic modulation, including chromatin remodeling and transcriptional regulation.


Storage & Stability

Store product at-20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



1. Zhang K, Chen Y, Zhang Z, et al. J Proteome Res. 2009, 8(2):900-906

2. Cheng Z, Tang Y, Chen Y, et al. Mol Cell Proteomics. 2009, 8(1):45-52.

3. Chen Y, Sprung R, Tang Y, et al. Mol Cell Proteomics. 2007, 6(5):812-819.


ELISA, WB; Not tested in other applications

 *WB=Western blotting



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