修饰抗体 Products
组蛋白赖氨酸丙酰化 (propionylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-propionyl-Histone H4 (Lys12) rabbit pAb

Cat#:PTM-206Ab Type:P

Ab Size:100 μlApplications:WB, Dot, ICC

Species:H M RPrice:¥ 3780.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by propionylated Histone H4(Lys12) peptide affinity chromatography. It specifically recognizes Histone H4 with propionylation at Lys12.  


Source/Purification

This product is produced by immunizing rabbits with a synthetic propionyl peptide corresponding to residues surrounding Lys12 of human histone H4. Antibodies are purified by protein A-conjugated agarose followed by propionylated histone H4(Lys12) peptide affinity chromatography  


Specificity

H4

Recommended Applications

ELISA, Dot, WB, ICC; Not tested in other applications

*WB=Western blotting;  ICC=Immunocytochemistry

Recommended antibody dilution

WB: 1:2000   ICC:1:200

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

 

Anti-propionyl-Histone H4 (Lys12) rabbit pAb

A. Dot blotting analysis on indicated amount of propionylated H4 peptide at Lys12 (lane 1), acetylated H4 peptide at Lys12 (lane 2), butyrylated H4 peptide at Lys12 (lane 3) and unmodified H4 peptide at Lys12 (lane 4) using propionyl-Histone H4 (Lys12) rabbit pAb. B. Western blotting analysis on 30 ug of crude proteins from HeLa whole cell lysates with (right) or without (left) sodium propionate treatment (10 mM, 4 hours) using propionyl-Histone  H4 (Lys12) rabbit pAb (1:1000).


Anti-propionyl-Histone H4 (Lys12) rabbit pAb

C. Immunocytochemistry analysis of Hela (Human cervix adenocarcinoma cell line), labeling H4K12pr with PTM-206 at 1/200 dilution. Cells were fixed with ice cold 100% methanol and permeabilized with 0.1% Triton X-100. Nuclei were stained with DAPI (blue).


Scientific Description

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Lysine propionylation (Kprop) is structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histione H3 at Lys12, propionylation of histone H4 at Lys12 may play a vital role in the epigenetic modulation, including chromatin remodeling and transcriptional regulation.  


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



ELISA, Dot, WB, ICC; Not tested in other applications

*WB=Western blotting; ICC= Immunocytochemistry


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