修饰抗体 Products
组蛋白赖氨酸丙酰化 (propionylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-propionyl-Histone H4 (Lys5) mouse mAb

Cat#:PTM-216Ab Type:M

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by propionylated Histone H4(Lys5) peptide affinity chromatography. It specifically recognizes Histone H4 with propionylation at Lys5.  


Source/Purification

This product is produced by immunizing mice with a synthetic propionyl peptide corresponding to residues surrounding Lys5 of human histone H4. Antibodies are purified by protein G-conjugated agarose followed by propionylated histone H4(Lys5) peptide affinity chromatography.  


Specificity

H4

Recommended Applications

ELISA, WB; Not tested in other applications  

*WB=Western blotting;  

Recommended antibody dilution: WB: 1:2000  


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

Anti-propionyl-Histone H4 (Lys5) mouse mAb

A. Propionyl-Histone H4 (Lys5) mouse mAb specificity was determined by peptide ELISA. The graph depicts the binding of the antibody to pre-coated propionyl-histone H4 (Lys5) peptide in the presence of increasing concentrations of various competitor peptides. As shown, only the propionyl-histone H4 (Lys5) peptide competed away binding of the antibody. 

  

B. Western blotting analysis on 30 ug of crude proteins from HeLa whole cell lysates with (lane 2) or without (lane 1) sodium propionate treatment (10 mM, 4 hours) using propionyl-Histone H4 (Lys5) mouse mAb (1:2000).  

 

Scientific Description

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Lysine propionylation (Kprop) is structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histione H3 at Lys12, propionylation of histone H4 at Lys5 may play a vital role in the epigenetic modulation, including chromatin remodeling and transcriptional regulation.  


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.  

 

**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.  


1. Zhang K, Chen Y, Zhang Z, et al.J Proteome Res. 2009, 8(2):900-906  

2. Cheng Z, Tang Y, Chen Y,et al.Mol Cell Proteomics. 2009, 8(1):45-52.  

3. Chen Y, Sprung R, Tang Y, et al. Mol Cell Proteomics. 2007, 6(5):812-819.  


ELISA, WB; Not tested in other applications  

*WB=Western blotting;  

Recommended antibody dilution: WB: 1:2000  


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