修饰抗体 Products
杭州景杰生物科技有限公司
Butyryl-Histone H3 (Lys18) mouse mAb

Cat#:PTM-331Ab Type:M

Ab Size:100 μlApplications:WB

Species:H, M, RPrice:¥ 3780.00


Product Description
Source/Purification

This product is produced by immunizing Balb/c mice with a synthetic butyryl peptide corresponding to residues surrounding Lys18 of human histone H3. Antibodies are purified by protein G-conjugated agarose followed by butyrylated histone H3 (Lys18) peptide affinity chromatography.


Specificity

H3

Recommended Applications

ELISA, Dot blot, Western blot.

Recommended antibody dilution: WB: 1:2000


Recommended antibody dilution

1: 2000

Important

For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. 

图片4.png

A.Dot blotting analysis on indicated amount of peptides using histone H3K18bu mouse mAb. The list of peptides is included in the table on the right.


B.Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (right) or without (left) treatment of sodium butyrate (30 mM, 4 hours) and Trichostatin A (500ng, 4 hours) (Figure B) or 30 μg of crude proteins from C2C12 whole cell lysates with (right) or without (left) treatment of sodium butyrate (30 mM, 4 hours) (Figure C) using butyryl-histone H3 (Lys18) mouse mAb (1: 2000).


Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine butyrylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone butyrylation marks either active promoters or potential enhancers. Butyrylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.


Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.



1. Zhang K, Chen Y, Zhang Z, et al. J Proteome Res. 2009, 8(2):900-906

2. Cheng Z, Tang Y, Chen Y, et al. Mol Cell Proteomics. 2009, 8(1):45-52.

3. Chen Y, Sprung R, Tang Y, et al. Mol Cell Proteomics. 2007, 6(5):812-819


ELISA, Dot blot, Western blot.

Recommended antibody dilution: WB: 1:2000


推荐产品 PRODUCTS
Copyright ©2018-2020 杭州景杰生物科技有限公司
犀牛云提供云计算服务
X
合作
交流
1

QQ设置

3

SKYPE 设置

4

阿里旺旺设置

等待加载动态数据...

等待加载动态数据...

5

电话号码管理

  • 400-100-1145
6

二维码管理

等待加载动态数据...

等待加载动态数据...

展开
合作交流
填写信息,我们的专属学术顾问将为您解答疑问!