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Anti-butyryl-Histone H2A (Lys5) rabbit pAb

Cat#:PTM-317Ab Type:P

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by butyrylated Histone H2A (Lys5) peptide affinity chromatography. It specifically recognizes Histone H2A with butyrylation at Lys5.


Source/Purification

This product is produced by immunizing rabbits with a synthetic butyryl peptide corresponding to residues surrounding Lys5 of human Histone H2A. Antibodies are purified by protein A-conjugated agarose followed by butyrylated Histone H2A (Lys5) peptide affinity chromatography.


Specificity

H2A

Recommended Applications

ELISA, WB. Not tested in other applications 

*WB=Western blotting


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

 

Anti-butyryl-Histone H2A (Lys5) rabbit pAb


A. Dot  blotting analysis on indicated amount of butyrylated H2A peptide at Lys5 (lane  1), acetylated H2A peptide at Lys5 (lane 2), 2ohibutyrylated H2A peptide at Lys5  (lane 3), 3ohbutyrylated H2A peptide at Lys5 (lane 4), acetylated H2A peptide  at Lys9 (lane 5), acetylated H2A peptide at Lys13 (lane 6), acetylated H2A  peptide at Lys15 (lane 7), acetylated H2A peptide at Lys36 (lane 8), crotonylated  H2A peptide at Lys36 (lane 9), 2ohibutyrylated H2A peptide at Lys36 (lane 10), 3ohbutyrylated  H2A peptide at Lys36 (lane 11), and unmodified H2A peptide at Lys5 (lane 12)  using butyryl-Histone H2A (Lys5) rabbit pAb.


B. Western blotting analysis on 30 μg of crude proteins from  HeLa whole cell lysates with (lane 2) or without (lane 1) treatment of sodium  butyrate (30 mM, 4 hours) using butyryl-Histone H2A (Lys5) rabbit pAb (1:2000).


Scientific Description

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine, structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. The reversible lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histione H4 at lysine 12, butyrylation of histone H2A at lysine 5 may play a vital role in the epigenetic modulation, including chromatin remodeling, and DNA transcriptional regulation.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



1. Zhang K, Chen Y, Zhang Z, et al. J Proteome Res. 2009, 8(2):900-906   

2. Cheng Z, Tang Y, Chen Y, et al. Mol Cell Proteomics. 2009, 8(1):45-52.   

3. Chen Y, Sprung R, Tang Y, et al. Mol Cell Proteomics. 2007, 6(5):812-819.  


ELISA, WB. Not tested in other applications 

*WB=Western blotting


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