修饰抗体 Products
组蛋白赖氨酸丁酰化 (butyrylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-butyryl-Histone H2A(Lys5) mouse mAb

Cat#:PTM-327Ab Type:M

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description
The mouse-derived antibody is purified with protein G-conjugated agarose followed by butyrylated Histone H2A at Lys5 peptide affinity chromatography. It specifically recognizes Histone H2A with butyrylation at Lys5.  
Source/Purification
This product is produced by immunizing Balb/c mice with a synthetic butyryl peptide corresponding to residues surrounding Lys5 of human histone H2A. Antibodies are purified by protein G-conjugated agarose followed by butyrylated histone H2A (Lys5) peptide affinity chromatography.   
Specificity

H2A

Recommended Applications

ELISA, WB; Not tested in other applications  

*WB=Western blotting;   


Recommended antibody dilution

WB: 1:2000

Important
For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking.  
Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications.     

 Anti-butyryl-Histone H2A(Lys5) mouse mAb 

 A. Dot blotting analysis on indicated amount of butyrylated H2A peptide at Lys5 (lane 1), 2ohibutyrylated H2A peptide at Lys5 (lane 2), 3ohbutyrylated H2A peptide at Lys5 (lane 3), succinylated H2A peptide at Lys9 (lane 4), 2ohibutyrylated H2A peptide at Lys9 (lane 5), 3ohbutyrylated H2A peptide at Lys9 (lane 6), acetylated H2A peptide at Lys36 (lane 7), crotonylated H2A peptide at Lys36 (lane 8), 2ohibutyrylated H2A peptide at Lys36 (lane 9), 3ohbutyrylated H2A peptide at Lys36 (lane 10), and unmodified H2A peptide at Lys5 (lane 11) using butyryl-Histone H2A (Lys5) mouse mAb.   

 

 B. Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (right) or without (left) treatment of sodium butyrate (30 mM, 4 hours) using butyryl-Histone H2A (Lys5) mouse mAb (1:2000).  

 

 C. Western blotting analysis on 30 μg of crude proteins from JK and BRL whole cell lysates using butyryl-Histone H2A (Lys5) mouse mAb (1:2000).  


Scientific Description
Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine butyrylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone butyrylation marks either active promoters or potential enhancers. Butyrylation of histone H2A at Lys5 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.  
Storage & Stability

The antibody is delivered in powder form. After dissolved with the antibody reconstitution buffer, please store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. 


**For research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



1. Zhang K, Chen Y, Zhang Z, et al. J Proteome Res. 2009, 8(2):900-906  
2. Cheng Z, Tang Y, Chen Y, et al. Mol Cell Proteomics. 2009, 8(1):45-52.  
3. Chen Y, Sprung R, Tang Y, et al. Mol Cell Proteomics. 2007, 6(5):812-819.  


ELISA, WB; Not tested in other applications  

*WB=Western blotting;   


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