修饰抗体 Products
杭州景杰生物科技有限公司
Anti-butyryl-Histone H2B (Lys12) rabbit pAb

Cat#:PTM-318Ab Type:P

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00

Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by butyrylated Histone H2B (Lys12) peptide affinity chromatography. It specifically recognizes Histone H2B with butyrylation at Lys12.


Source/Purification

This product is produced by immunizing rabbits with a synthetic butyryl peptide corresponding to residues surrounding Lys12 of human Histone H2B. Antibodies are purified by protein A-conjugated agarose followed by butyrylated Histone H2B (Lys12) peptide affinity chromatography.


Specificity

H2B

Recommended Applications

ELISA, WB, IP; Not tested in other applications

*WB=Western blotting; IP= Immunoprecipation


 
 

Recommended antibody dilution

WB: 1:2000 IP: 1:100

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

 Anti-butyryl-Histone H2B (Lys12) rabbit pAb  

A. Dot  blotting analysis on indicated amount of butyrylated H2B peptide at Lys12 (lane  1), butyrylated H2B peptide at Lys5 (lane 2), crotonylated H2B peptide at Lys5 (lane  3), mono-methylated H2B peptide at Lys5 (lane 4), 2ohibutyrylated H2B peptide  at Lys5 (lane 5), 3ohbutyrylated H2B peptide at Lys5 (lane 6), butyrylated H2B peptide  at Lys11 (lane 7), crotonylated H2B peptide at Lys11 (lane 8) , 3ohbutyrylated H2B  peptide at Lys11 (lane 9) , acetylated H2B peptide at Lys12 (lane 10) ,  crotonylated H2B peptide at Lys12 (lane 11), 2ohibutyrylated H2B peptide at Lys12  (lane 12), 3ohbutyrylated H2B peptide at Lys12 (lane 13), butyrylated H2B peptide  at Lys15 (lane 14), butyrylated H2B peptide at Lys16 (lane 15) , crotonylated H2B  peptide at Lys16(lane 16) , and unmodified H2B peptide at Lys12 (lane 17) using  butyryl-Histone H2B (Lys12) rabbit pAb.


B. Western blotting analysis on 30 μg of crude proteins from  HeLa whole cell lysates with (lane 2) or without (lane 1) treatment of sodium  butyrate (30 mM, 4 hours) using butyryl-Histone H2B (Lys12) rabbit pAb (1:2000).


Scientific Description

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine, structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. The reversible lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histione H4 at lysine 12, butyrylation of histone H2B at lysine 12 may play a vital role in the epigenetic modulation, including chromatin remodeling, and DNA transcriptional regulation.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


Research purposes only.Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



1. Zhang K, Chen Y, Zhang Z, et alJ Proteome Res. 2009,  8(2):900-906  

2. Cheng Z, Tang Y, Chen Y, et al. Mol Cell Proteomics. 2009, 8(1):45-52.  

3. Chen Y, Sprung R, Tang Y, et alMol Cell Proteomics. 2007, 6(5):812-819.  


ELISA, WB, IP; Not tested in other applications

*WB=Western blotting; IP= Immunoprecipation

推荐产品 PRODUCTS
Copyright ©2018-2020 杭州景杰生物科技有限公司
犀牛云提供云计算服务
X
合作
交流
1

QQ设置

3

SKYPE 设置

4

阿里旺旺设置

等待加载动态数据...

等待加载动态数据...

5

电话号码管理

  • 400-100-1145
6

二维码管理

等待加载动态数据...

等待加载动态数据...

展开
合作交流
填写信息,我们的专属学术顾问将为您解答疑问!