修饰抗体 Products
杭州景杰生物科技有限公司
Anti-butyryl-Histone H2B (Lys15) mouse mAb

Cat#:PTM-324Ab Type:M

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by butyrylated Histone H2B (Lys15) peptide affinity chromatography. It specifically recognizes Histone H2B with butyrylation at Lys15.

 

Source/Purification

This product is produced by immunizing mice with a synthetic butyryl peptide corresponding to residues surrounding Lys15 of human Histone H2B. Antibodies are purified by protein G-conjugated agarose followed by butyrylated Histone H2B (Lys15) peptide affinity chromatography.  


Specificity

H2B

Recommended Applications

ELISA, WB. Not tested in other applications  

*WB=Western blotting  


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user.  

 Anti-butyryl-Histone H2B (Lys15) mouse mAb

AButyryl-Histone H2B (Lys15) mouse mAb specificity was determined by peptide ELISA. The graph depicts the binding of the antibody to pre-coated butyryl-histone H2B (Lys15) peptide in the presence of increasing concentrations of various competitor peptides. As shown, only the butyryl-histone H2B (Lys15) peptide competed away binding of the antibody.


B. Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (right) or without (left) treatment of sodium butyrate (30 mM, 4 hours) using butyryl-Histone H2B (Lys15) mouse mAb (1:2000).


Scientific Description

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine, structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. The reversible lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histione H4 at lysine 12, butyrylation of histone H2B at lysine 15 may play a vital role in the epigenetic modulation, including chromatin remodeling, and DNA transcriptional regulation.

 

Storage & Stability

Store product at -20oC.


ELISA, WB. Not tested in other applications  

*WB=Western blotting  


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