修饰抗体 Products
组蛋白赖氨酸丁酰化 (butyrylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-butyryl-Histone H2B (Lys16) rabbit pAb

Cat#:PTM-319Ab Type:P

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by butyrylated Histone H2B (Lys16) peptide affinity chromatography. It specifically recognizes Histone H2B with butyrylation at Lys16.  

Source/Purification

This product is produced by immunizing rabbits with a synthetic butyryl peptide corresponding to residues surrounding Lys16 of human Histone H2B. Antibodies are purified by protein A-conjugated agarose followed by butyrylated Histone H2B (Lys16) peptide affinity chromatography.  

Specificity

H2B

Recommended Applications

ELISA, WB. Not tested in other applications   

*WB=Western blotting  


Recommended antibody dilution

WB: 1:2000

Important

 For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

 Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

 Anti-butyryl-Histone H2B (Lys16) rabbit pAb
 
  

 A. Dot  blotting analysis on indicated amount of butyrylated H2B peptide at Lys16 (lane  1), butyrylated H2B peptide at Lys11 (lane 2), crotonylated H2B peptide at Lys11  (lane 3), 3ohbutyrylated H2B peptide at Lys11 (lane 4), acetylated H2B peptide  at Lys12 (lane 5), butyrylated H2B peptide at Lys12 (lane 6), crotonylated H2B  peptide at Lys12 (lane 7), 2ohibutyrylated H2B peptide at Lys12 (lane 8), 3ohbutyrylated  H2B peptide at Lys12 (lane 9), acetylated H2B peptide at Lys15 (lane 10),  butyrylated H2B peptide at Lys15 (lane 11), crotonylated H2B peptide at Lys15  (lane 12), 3ohbutyrylated H2B peptide at Lys15 (lane 13), acetylated H2B peptide  at Lys16 (lane 14), crotonylated H2B peptide at Lys16 (lane 15) , 3ohbutyrylated  H2B peptide at Lys16(lane 16), acetylated H2B peptide at Lys20 (lane 17), butyrylated  H2B peptide at Lys20 (lane 18), crotonylated H2B peptide at Lys20 (lane 19), 2ohibutyrylated  H2B peptide at Lys20 (lane 20), 3ohbutyrylated H2B peptide at Lys20 (lane 21), acetylated  H2B peptide at Lys23 (lane 22), butyrylated H2B peptide at Lys23 (lane 23),  crotonylated H2B peptide at Lys23 (lane 24), 2ohibutyrylated H2B peptide at Lys23  (lane 25), 3ohbutyrylated H2B peptide at Lys23 (lane 26), and unmodified H2B peptide  at Lys16 (lane 27) using butyryl-Histone H2B (Lys16) rabbit pAb.  


 B. Western blotting analysis on 30 μg of crude proteins from  HeLa whole cell lysates with (lane 2) or without (lane 1) treatment of sodium  butyrate (30 mM, 4 hours) using butyryl-Histone H2B (Lys16) rabbit pAb (1:2000).  

Scientific Description

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, and ubiquitylation, etc. Butyrylation of lysine, structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. The reversible lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histione H4 at lysine 12, butyrylation of histone H2B at lysine 16 may play a vital role in the epigenetic modulation, including chromatin remodeling, and DNA transcriptional regulation.  


Storage & Stability

Store product at -20oC.



ELISA, WB. Not tested in other applications   

*WB=Western blotting  


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