修饰抗体 Products
组蛋白赖氨酸丁酰化 (butyrylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-butyryl-Histone H2B (Lys20) rabbit pAb

Cat#:PTM-304Ab Type:P

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by butyrylated Histone H2B(Lys20) peptide affinity chromatography. It specifically recognizes Histone H2B with butyrylation at Lys20.

  

Source/Purification

This product is produced by immunizing rabbits with a synthetic butyryl peptide corresponding to residues surrounding Lys20 of human histone H2B. Antibodies are purified by protein A-conjugated agarose followed by butyrylated histone H2B (Lys20) peptide affinity chromatography.  


Specificity

H2B

Recommended Applications

ELISA, WB, IP; Not tested in other applications

*WB=Western blotting; IP= Immunoprecipation


Recommended antibody dilution

WB: 1:2000 IP: 1:50

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.   Anti-butyryl-Histone H2B (Lys20) rabbit pAb

A. Dot blotting analysis on indicated amount of butyrylated H2B peptide at Lys20 (lane  1), acetylated H2B peptide at Lys12 (lane 2), crotonylated H2B peptide at Lys12  (lane 3), acetylated H2B peptide at Lys15 (lane 4), crotonylated H2B peptide at  Lys16 (lane 5), acetylated H2B peptide at Lys20 (lane 6), crotonylated H2B  peptide at Lys20 (lane 7), crotonylated H2B peptide at Lys23 (lane 8) and  unmodified H2B peptide at Lys20 (lane 9) using butyryl-Histone H2B (Lys20) rabbit  pAb.


B. Western blotting analysis on 30 μg of crude proteins from  HeLa whole cell lysates with (lane 2) or without (lane 1) treatment of sodium  butyrate (30 mM, 4 hours) using butyryl-Histone H2B (Lys20) rabbit pAb (1:2000).  


Scientific Description

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine, structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. The reversible lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histione H4 at lysine 12, butyrylation of histone H2B at lysine 20 may play a vital role in the epigenetic modulation, including chromatin remodeling, and DNA transcriptional regulation.  

 

Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



ELISA, WB, IP; Not tested in other applications

*WB=Western blotting; IP= Immunoprecipation

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