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Anti-butyryl-Histone_H2B_(Lys23)_rabbit_pAb

Cat#:PTM-321Ab Type:P

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description

This product is produced by immunizing rabbits with a synthetic butyryl peptide corresponding to residues surrounding Lys23 of human Histone H2B. Antibodies are purified by protein A-conjugated agarose followed by butyrylated Histone H2B (Lys23) peptide affinity chromatography. 


Source/Purification

This product is produced by immunizing rabbits with a synthetic butyryl peptide corresponding to residues surrounding Lys23 of human Histone H2B. Antibodies are purified by protein A-conjugated agarose followed by butyrylated Histone H2B (Lys23) peptide affinity chromatography.

 

Specificity

H2B

Recommended Applications

ELISA, WB. Not tested in other applications

*WB=Western blotting


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking.

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user

Anti-butyryl-Histone_H2B_(Lys23)_rabbit_pAb

A. Dot blotting analysis on indicated amount of butyrylated H2B peptide at Lys23 (lane 1), butyrylated H2B peptide at Lys15 (lane 2), crotonylated H2B peptide at Lys15 (lane 3), acetylated H2B peptide at Lys16 (lane 4), butyrylated H2B peptide at Lys16 (lane 5), crotonylated H2B peptide at Lys16 (lane 6), acetylated H2B peptide at Lys20 (lane 7) , butyrylated H2B peptide at Lys20 (lane 8), crotonylated H2B peptide at Lys20 (lane 9), 2ohibutyrylated H2B peptide at Lys20 (lane 10), acetylated H2B peptide at Lys23 (lane 11), crotonylated H2B peptide at Lys23(lane 12), 2ohibutyrylated H2B peptide at Lys23 (lane 13), succinylated H2B peptide at Lys34(lane14), acetylated H2B peptide at Lys24 (lane 15), 2ohibutyrylated H2B peptide at Lys24 (lane 16), crotonylated H2B peptide at Lys34 (lane 17), 2ohibutyrylated H2B peptide at Lys34 (lane 18), and unmodified H2B peptide at Lys23 (lane 19) using butyryl-Histone H2B (Lys23) rabbit pAb. 

B. Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (lane 2) or without (lane 1) treatment of sodium butyrate (30 mM, 4 hours) using butyryl-Histone H2B (Lys23) rabbit pAb (1:2000).

Scientific Description

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine, structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. The reversible lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histione H4 at lysine 12, butyrylation of histone H2B at lysine 23 may play a vital role in the epigenetic modulation, including chromatin remodeling, and DNA transcriptional regulation.


Storage & Stability

Store product at -20°C. Avoid repeated freeze/thaw cycles. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt. 


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


1. Zhang K, Chen Y, Zhang Z, et al. J Proteome Res. 2009, 8(2):900-906
2. Cheng Z, Tang Y, Chen Y, et al. Mol Cell Proteomics. 2009, 8(1):45-52.
3. Chen Y, Sprung R, Tang Y, et al. Mol Cell Proteomics. 2007, 6(5):812-819.


ELISA, WB. Not tested in other applications

*WB=Western blotting


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