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Anti-butyryl-Histone H2B(Lys16) mouse mAb

Cat#:PTM-322Ab Type:M

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00

Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by propionylated Histone H4 (Lys8) peptide affinity chromatography. It specifically recognizes Histone H4 with propionylation at Lys8.  


Source/Purification

This product is produced by immunizing Balb/c mice with a synthetic propionyl peptide corresponding to residues surrounding Lys8 of human histone H4. Antibodies are purified by protein G-conjugated agarose followed by propionylated histone H4 (Lys8) peptide affinity chromatography.   


Specificity

H2B

Recommended Applications
ELISA, WB; Not tested in other applications  
*WB=Western blotting;   
Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user.  

 

Anti-butyryl-Histone H2B(Lys16) mouse mAb

 A. Dot blotting analysis on indicated amount of propionylated H4 peptide at Lys8 (lane 1), acetylated H4 peptide at Lys5 (lane 2), propionylated H4 peptide at Lys5 (lane 3), butyrylated H4 peptide at Lys5 (lane 4), crotonylated H4 peptide at Lys5 (lane 5), butyrylated H4 peptide at Lys8 (lane 6), crotonylated H4 peptide at Lys8 (lane 7), acetylated H4 peptide at Lys12 (lane 8), propionylated H4 peptide at Lys12 (lane 9), butyrylated H4 peptide at Lys12 (lane 10), crotonylated H4 peptide at Lys12 (lane 11), and unmodified H4 peptide at Lys8 (lane 12) using propionyl-Histone H4 (Lys8) mouse mAb. 


  B. Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (right) or without (left) treatment of sodium butyrate (30 mM, 4 hours) and Trichostatin A (500ng, 4 hours) using propionyl-Histone H4 (Lys8) mouse mAb (1:1000).


  C. Western blotting analysis on 30 μg of crude proteins from C2C12 whole cell lysates with (right) or without (left) sodium butyrate treatment (30 mM, 4 hours) using propionyl-Histone H4 (Lys8) mouse mAb (1:1000).
  


Scientific Description

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Lysine propionylation (Kprop) is structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. Lysine propionylation is abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histone H3 at Lys12, propionylation of histone H4 at Lys8 may play a vital role in the epigenetic modulation, including chromatin remodeling and transcriptional regulation.  


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.  

 

**Research purposes only.Not intended to be used for therapeutic or diagnostic purposes in humans or animals. 


1. Zhang K, Chen Y, Zhang Z, et al. J Proteome Res. 2009, 8(2):900-906  
2. Cheng Z, Tang Y, Chen Y,et al. Mol Cell Proteomics. 2009, 8(1):45-52.  
3. Chen Y, Sprung R, Tang Y, et al. Mol Cell Proteomics. 2007, 6(5):812-819.  


ELISA, WB; Not tested in other applications  
*WB=Western blotting;   


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