修饰抗体 Products
杭州景杰生物科技有限公司
Anti-butyryl-Histone H2B(Lys23) mouse mAb

Cat#:PTM-328Ab Type:M

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description
Source/Purification

This product is produced by immunizing Balb/c mice with a synthetic butyryl peptide corresponding to residues surrounding Lys23 of human histone H2B. Antibodies are purified by protein G-conjugated agarose followed by butyrylated histone H2B (Lys23) peptide affinity chromatography.


Specificity

H2B

Recommended Applications
ELISA, Dot blot, Western blot.   
Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

 Anti-butyryl-Histone H2B(Lys23) mouse mAb

A. Dot blotting analysis on indicated amount of peptides using histone H2BK23bu mouse mAb. The list of peptides is included in the table on the right.  
B. Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (right) or without (left) treatment of sodium butyrate (30 mM, 4 hours) (Figure B) using butyryl-histone H2B (Lys23) mouse mAb(1: 2000).   
Scientific Description
Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine butyrylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone butyrylation marks either active promoters or potential enhancers. Butyrylation of histone H2A at Lys5 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.  
Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20℃.



ELISA, Dot blot, Western blot.   

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