修饰抗体 Products
组蛋白赖氨酸丁酰化 (butyrylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-butyryl-Histone H3 (Lys18) rabbit pAb

Cat#:PTM-306Ab Type:P

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description
The rabbit-derived antibody is purified with protein A-conjugated agarose followed by butyrylated Histone H3 (Lys18) peptide affinity chromatography. It specifically recognizes Histone H3 with butyrylation at Lys18.  
Source/Purification
This product is produced by immunizing rabbits with a synthetic butyryl peptide corresponding to residues surrounding Lys18 of human Histone H3. Antibodies are purified by protein A-conjugated agarose followed by butyrylated Histone H3 (Lys18) peptide affinity chromatography.  
Specificity

H3

Recommended Applications

ELISA, WB, IP; Not tested in other applications

*WB=Western blotting; IP= Immunoprecipation


Recommended antibody dilution

WB: 1:2000 IP: 1:50

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

 

  Anti-butyryl-Histone H3 (Lys18) rabbit pAb

 

A. Dot blotting analysis on indicated amount of butyrylated H3  peptide at Lys18 (lane 1), acetylated H3 peptide at Lys14 (lane 2),  propionylated H3 peptide at Lys14 (lane 3), 2ohibutyrylated H3 peptide at Lys14  (lane 4), 3ohbutyrylated H3 peptide at Lys14 (lane 5), acetylated H3 peptide at  Lys18 (lane 6), propionylated H3 peptide at Lys18 (lane 7) , 3ohbutyrylated H3  peptide at Lys18 (lane 8), crotonylated H3 peptide at Lys18 (lane 9),  propionylated H3 peptide at Lys23 (lane 10), 2ohibutyrylated H3 peptide at  Lys23 (lane 11), 3ohbutyrylated H3 peptide at Lys23 (lane 12), crotonylated H3  peptide at Lys23 (lane 13), and unmodified H3 peptide at Lys18 (lane 14) using  butyryl-Histone H3 (Lys18) rabbit pAb.


B. Western blotting analysis on 30 μg of crude proteins from  HeLa whole cell lysates with (lane 2) or without (lane 1) treatment of sodium  butyrate (30 mM, 4 hours) using butyryl-Histone H3 (Lys18) rabbit pAb (1:2000).  


Scientific Description
Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine, structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. The reversible lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histione H4 at lysine 12, butyrylation of histone H3 at lysine 18 may play a vital role in the epigenetic modulation, including chromatin remodeling, and DNA transcriptional regulation.  
Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


ELISA, WB, IP; Not tested in other applications

*WB=Western blotting; IP= Immunoprecipation


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