修饰抗体 Products
杭州景杰生物科技有限公司
Anti-butyryl-Histone H3 (Lys23) mouse mAb

Cat#:PTM-307Ab Type:M

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by butyrylated Histone H3 at Lys23 peptide affinity chromatography. It specifically recognizes Histone H3 with butyrylation at Lys23.  


Source/Purification

This product is produced by immunizing mice with a synthetic butyryl peptide corresponding to residues surrounding Lys23 of human histone H3. Antibodies are purified by protein G-conjugated agarose followed by butyrylated histone H3 (Lys23) peptide affinity chromatography


Specificity

H3

Recommended Applications

ELISA, WB; Not tested in other applications 

*WB=Western blotting


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting,  incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1%  Tween-20 for two hours at room temperature with gentle shaking.  

Use at an assay dependent  concentration. Optimal dilutions/concentrations should be determined by the end  user.  

 Anti-butyryl-Histone H3 (Lys23) mouse mAb  

A. Dot  blotting analysis on indicated amount of butyrylated H3 peptide at Lys23 (lane  1), acetylated H3 peptide at Lys18 (lane 2), propionylated H3 peptide at Lys18  (lane 3), butyrylated H3 peptide at Lys18 (lane 4), crotonylated H3 peptide at Lys18  (lane 5), mono-methylated H3 peptide at Lys18 (lane 6), acetylated H3  peptide at Lys23 (lane 7), propionylated H3 peptide at Lys23 (lane 8), crotonylated H3 peptide at Lys23 (lane 9), mono-methylated H3  peptide at Lys23 (lane 10), acetylated H3 peptide at Lys27 (lane 11), crotonylated H3 peptide at Lys27 (lane 12), mono-methylated H3  peptide at Lys27 (lane 13), di-methylated H3 peptide at Lys27 (lane 14),  tri-methylated H3 peptide at Lys27 (lane 15), and unmodified H3 peptide  at Lys23 (lane 16) using butyryl-Histone H3 (Lys23) mouse mAb.


B. Western blotting analysis  on 30 μg of crude proteins from HeLa whole cell lysates with (lane 2) or without (lane 1) treatment of sodium butyrate (30 mM, 4 hours) using  butyryl-Histone H3 (Lys23) mouse mAb (1:2000).  


Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine butyrylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine butyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone butyrylation marks either active promoters or potential enhancers. Butyrylation of histone H3 at Lys23 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.  



Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw cycles. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.


**Research purposes only. Not intended  to be used for therapeutic or diagnostic purposes in humans or animals. 


ELISA, WB; Not tested in other applications 

*WB=Western blotting


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