修饰抗体 Products
组蛋白赖氨酸丁酰化 (butyrylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-butyryl-Histone H3 (Lys27) rabbit pAb

Cat#:PTM-315Ab Type:P

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by butyrylated Histone H3 (Lys27) peptide affinity chromatography. It specifically recognizes Histone H3 with butyrylation at Lys27.



Source/Purification

This product is produced by immunizing rabbits with a synthetic butyryl peptide corresponding to residues surrounding Lys27 of human Histone H3. Antibodies are purified by protein A-conjugated agarose followed by butyrylated Histone H3 (Lys27) peptide affinity chromatography.  



Specificity

H3

Recommended Applications

ELISA, WB. Not tested in other applications 

*WB=Western blotting


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

 Anti-butyryl-Histone H3 (Lys27) rabbit pAb 

A. Dot  blotting analysis on indicated amount of butyrylated H3 peptide at Lys27 (lane  1), acetylated H3 peptide at Lys18 (lane 2), propionylated H3 peptide at Lys18 (lane  3), crotonylated H3 peptide at Lys18 (lane 4), 2ohibutyrylated H3 peptide at Lys18  (lane 5), 3ohbutyrylated H3 peptide at Lys18 (lane 6), acetylated H3 peptide at  Lys23 (lane 7), propionylated H3 peptide at Lys23 (lane 8) , butyrylated H3  peptide at Lys23 (lane 9) , succinylated H3 peptide at Lys23 (lane 10) ,  crotonylated H3 peptide at Lys23(lane 11), 2ohibutyrylated H3 peptide at Lys23  (lane 12), 3ohbutyrylated H3 peptide at Lys23 (lane 13), acetylated H3 peptide  at Lys27 (lane 14), succinylated H3 peptide at Lys27 (lane 15) , crotonylated  H3 peptide at Lys27(lane 16), 2ohibutyrylated H3 peptide at Lys27 (lane 17),  3ohbutyrylated H3 peptide at Lys27 (lane1 8), acetylated H3 peptide at Lys36  (lane 19), crotonylated H3 peptide at Lys36 (lane 20), 2ohibutyrylated H3  peptide at Lys36 (lane 21), and unmodified H3 peptide at Lys27 (lane 22) using  butyryl-Histone H3 (Lys27) rabbit pAb.


B. Western blotting analysis on 30 μg of crude proteins from  HeLa whole cell lysates with (lane 2) or without (lane 1) treatment of sodium  butyrate (30 mM, 4 hours) using butyryl-Histone H3 (Lys27) rabbit pAb (1:2000).  

 

Scientific Description

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine, structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. The reversible lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histione H4 at lysine 12, butyrylation of histone H3 at lysine 27 may play a vital role in the epigenetic modulation, including chromatin remodeling, and DNA transcriptional regulation.  


 

Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



ELISA, WB. Not tested in other applications 

*WB=Western blotting


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