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Anti-butyryl-Histone H3(Lys115) rabbit pAb

Cat#:PTM-325Ab Type:P

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by butyrylated Histone H3 (Lys115) peptide affinity chromatography. It specifically recognizes Histone H3 butyrylated at Lys115 or H4 butylated at Lys77.  


Source/Purification

This product is produced by immunizing rabbits with a synthetic butyryl peptide corresponding to residues surrounding Lys115 of human Histone H3. Antibodies are purified by protein A-conjugated agarose followed by butyrylated Histone H3 (Lys115) peptide affinity chromatography. 

  

Specificity

H3

Recommended Applications

ELISA, WB. Not tested in other applications  

*WB=Western blotting

 

Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user.   

Anti-butyryl-Histone H3(Lys115) rabbit pAb

A.Dot blotting analysis on indicated amount of butyrylated H3 peptide at Lys115 (lane 1), butyrylated H4 peptide at Lys77 (lane 2), acetylated H3 peptide at Lys115 (lane 3), acetylated H3 peptide at Lys122 (lane 4), butyrylated H3 peptide at Lys122 (lane 5), crotonylated H3 peptide at Lys122 (lane 6),  and unmodified H3 peptide at Lys115 (lane 7) using butyryl-Histone H3 (Lys115)/ H4 (Lys77) rabbit pAb.  

 

B. Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (right) or without (left) treatment of sodium butyrate (30 mM, 4 hours) using butyryl- Histone H3 (Lys115)/ H4 (Lys77) rabbit pAb (1:2000).  

 

C. Western blotting analysis on 30 μg of crude proteins from C2C12 whole cell lysates with (right) or without (left) treatment of sodium butyrate (30 mM, 4 hours) using butyryl- Histone H3 (Lys115)/ H4 (Lys77) rabbit pAb (1:2000).  

 

Scientific Description

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine, structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. The reversible lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histione H4 at lysine 12, butyrylation of histone H3 at lysine 115 may play a vital role in the epigenetic modulation, including chromatin remodeling, and DNA transcriptional regulation.  


Storage & Stability

The antibody is delivered in powder form. After dissolved with the antibody reconstitution buffer, please store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. 


**For research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


1. Zhang K, Chen Y, Zhang Z, et al. J Proteome Res. 2009, 8(2):900-906  

2. Cheng Z, Tang Y, Chen Y, et al. Mol Cell Proteomics. 2009, 8(1):45-52.  

3. Chen Y, Sprung R, Tang Y, et al. Mol Cell Proteomics. 2007, 6(5):812-819.  


ELISA, WB. Not tested in other applications  

*WB=Western blotting

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