修饰抗体 Products
组蛋白赖氨酸丁酰化 (butyrylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-butyryl-Histone H4 (Lys12) mouse mAb

Cat#:PTM-314Ab Type:M

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by butyrylated Histone H4 peptide at Lys12 affinity chromatography. It specifically recognizes Histone H4 with butyrylation at Lys12.  


Source/Purification

This product is produced by immunizing mice with a synthetic butyryl peptide corresponding to residues surrounding Lys12 of human histone H4. Antibodies are purified by protein G-conjugated agarose followed by butyrylated histone H4 (Lys12) peptide affinity chromatography.  

Specificity

H4

Recommended Applications

 ELISA, WB, IP; Not tested in other applications 

 *WB=Western blotting; IP= Immunoprecipation


Recommended antibody dilution

WB: 1:2000 IP: 1:25

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

 

 Anti-butyryl-Histone H4 (Lys12) mouse mAb  

A. Dot blotting analysis on indicated amount of butyrylated H4 peptide at Lys12 (lane  1), acetylated H4 peptide at Lys12 (lane 2), propionylated H4 peptide at Lys12  (lane 3), crotonylated H4 peptide at Lys12 (lane 4), acetylated H4 peptide at  Lys8 (lane 5), butyrylated H4 peptide at Lys8 (lane 6) , crotonylated H4  peptide at Lys8 (lane 7), acetylated H4 peptide at Lys16 (lane 8), propionylated  H4 peptide at Lys16 (lane 9), butyrylated H4 peptide at Lys16 (lane 10), crotonylated  H4 peptide at Lys16 (lane 11) and unmodified H4 peptide at Lys12 (lane 12) using  butyryl-Histone H4 (Lys12) mouse mAb.

 

B. Western blotting analysis on 30 μg of crude proteins from  HeLa whole cell lysates with (lane 2) or without (lane 1) treatment of sodium  butyrate (30 mM, 4 hours) using butyryl-Histone H4 (Lys12) mouse mAb (1:2000).  

 

Scientific Description

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Butyrylation of lysine, structurally similar to lysine acetylation, is a newly identified reversible modification controlling protein activity. The reversible lysine butyrylation are abundant in both prokaryotes and eukaryotes and has been found in wide ranges of proteins including histones and non-histone substrates, such as p53. Similar to acetylation of histione H4 at lysine 12, butyrylation of histone H4 at lysine 12 may play a vital role in the epigenetic modulation, including chromatin remodeling, and DNA transcriptional regulation.  


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt. 

 

**Research purposes only. Not intended  to be used for therapeutic or diagnostic purposes in humans or animals.  


 ELISA, WB, IP; Not tested in other applications 

 *WB=Western blotting; IP= Immunoprecipation


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