修饰抗体 Products
组蛋白赖氨酸巴豆酰化 (crotonylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-crotonyl-Histone H3 (Lys18) mouse mAb

Cat#:PTM-540Ab Type:M

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description
Source/Purification

This product is produced by immunizing mice with a synthetic histone H3 peptide which is crotonylated at Lys18. Antibodies are purified by protein G-conjugated agarose.

 

Specificity

H3

Recommended Applications

ELISA, Dot blot, Western blot.   


Recommended antibody dilution

WB: 1:2000

Important

 For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking.  

 Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. 

捕获12.JPG

A.Dot blotting analysis on indicated amount of peptides using histone H3K18cr mouse mAb. The list of peptides is included in the table on the right.  

捕获13.JPG

B.Western blotting analysis on 30 μg of crude proteins from human HeLa whole cell lysates with (right) or without (left) treatment of crotonic acid (100 ng/ml, 18hr) (Figure B) and 30 μg of crude proteins from JK and BRL whole cell lysates (Figure C) using crotonyl-histone H3 (Lys18) mouse mAb(1: 2000).   


Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine crotonylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.      

 
Storage & Stability

The antibody is delivered in powder form.Before use, please read carefully the Reconstitution and Storage Instructions. After dissolved with the antibody reconstitution buffer .please store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt.


Zhang K, Chen Y, Zhang Z, et al. J Proteome Res. 20098(2):900-906  

Cheng Z, Tang Y, Chen Y, et al. Mol Cell Proteomics. 20098(1):45-52.  

Chen Y, Sprung R, Tang Y, et alMol Cell Proteomics. 20076(5):812-819.  


ELISA, Dot blot, Western blot.   


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