修饰抗体 Products
组蛋白赖氨酸巴豆酰化 (crotonylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-crotonyl-Histone H3 (Lys9) mouse mAb

Cat#:PTM-539Ab Type:M

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by crotonylated Histone H3 at Lys9 peptide affinity chromatography. It specifically recognizes Histone H3 with crotonylation at Lys9.  


Source/Purification

This product is produced by immunizing mice with a synthetic crotonyl peptide corresponding to residues surrounding Lys9 of human histone H3. Antibodies are purified by protein G-conjugated agarose followed by crotonylated histone H3 (Lys9) peptide affinity chromatography.  


Specificity

H3

Recommended Applications

ELISA, WB; Not tested in other applications  

*WB=Western blotting;  


Recommended antibody dilution

WB: 1:2000

Important

 For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking.  

 Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. 

Anti-crotonyl-Histone H3 (Lys9) mouse mAb

A. Dot blotting analysis on indicated amount of crotonylated H3 peptide at Lys9 (lane 1), acetylated H3 peptide at Lys4 (lane 2), crotonylated H3 peptide at Lys4 (lane 3), 2ohibutyrylated H3 peptide at Lys4 (lane 4), 3ohbutyrylated H3 peptide at Lys4 (lane 5), acetylated H3 peptide at Lys9 (lane 6), butyrylated H3 peptide at Lys9 (lane 7), crotonylated H3 peptide at Lys9 (lane 8), 2ohibutyrylated H3 peptide at Lys9 (lane 9), 3ohbutyrylated H3 peptide at Lys9 (lane 10), acetylated H3 peptide at Lys14 (lane 11), propionylated H3 peptide at Lys14 (lane 12), butyrylated H3 peptide at Lys14 (lane 13), succinylated H3 peptide at Lys14 (lane 14), crotonylated H3 peptide at Lys14 (lane 15), 2ohibutyrylated H3 peptide at Lys14 (lane 16), 3ohbutyrylated H3 peptide at Lys14 (lane 17) and unmodified H3 peptide at Lys14 (lane 18) using crotonyl-Histone H3 (Lys9) mouse mAb.  


B. Western blotting analysis on 30 μg of crude proteins from human HeLa whole cell lysates with (Lane 2) or without (Lane 1) treatment of crotonic acid (100 ng/ml, 18hr) using crotonyl-Histone H3 (Lys9) mouse mAb (1:2000)  


Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine crotonylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H3 at Lys9 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.  


Storage & Stability

The antibody is delivered by powder. After dissolved with the antibody constitution buffer, please store it at-20oC.



1. Zhang K, Chen Y, Zhang Z, et al. J Proteome Res. 2009, 8(2):900-906  

2. Cheng Z, Tang Y, Chen Y, et al. Mol Cell Proteomics. 2009, 8(1):45-52.  

3. Chen Y, Sprung R, Tang Y, et al. Mol Cell Proteomics. 2007, 6(5):812-819.  


ELISA, WB; Not tested in other applications  

*WB=Western blotting;  



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