修饰抗体 Products
组蛋白赖氨酸巴豆酰化 (crotonylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-crotonyl-Histone H2B (Lys20) mouse mAb

Cat#:PTM-534Ab Type:M

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by crotonylated Histone H2B at Lys20 peptide affinity chromatography. It specifically recognizes Histone H2B with crotonylation at Lys20.  


Source/Purification

This product is produced by immunizing mice with a synthetic crotonyl peptide corresponding to residues surrounding Lys20 of human histone H2B. Antibodies are purified by protein G-conjugated agarose followed by crotonylated histone H2B (Lys20) peptide affinity chromatography.  


Specificity

H2B

Recommended Applications

ELISA, WB; Not tested in other applications 

*WB=Western blotting


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

Anti-crotonyl-Histone H2B (Lys20) mouse mAb

A. Dot  blotting analysis on indicated amount of crotonylated H2B peptide at Lys20  (lane 1), acetylated H2B peptide at Lys15 (lane 2), crotonylated H2B peptide at  Lys15 (lane 3), 3ohbutyrylated H2B peptide at Lys15 (lane 4), acetylated H2B  peptide at Lys16 (lane 5), crotonylated H2B peptide at Lys16 (lane 6), 3ohbutyrylated H2B peptide at Lys16 (lane 7), acetylated H2B  peptide at Lys20 (lane 8), butyrylated H2B peptide at Lys20 (lane 9), 2ohibutyrylated H2B peptide at Lys20 (lane 10), 3ohbutyrylated H2B  peptide at Lys20 (lane 11), crotonylated H2B peptide at Lys23 (lane 12),  2ohibutyrylated H2B peptide at Lys23 (lane 13), 3ohbutyrylated H2B  peptide at Lys23 (lane 14), 2ohibutyrylated H2B peptide at Lys24 (lane 15), 3ohbutyrylated  H2B peptide at Lys24 (lane 16), and unmodified H2B peptide at Lys20  (lane 17) using crotonyl-Histone H2B (Lys20) mouse mAb.


B. Western blotting analysis on 30 μg of crude  proteins from HeLa, C2C12, COS-7 whole cell lysates, and  200 ng recombinant histone H2B protein using crotonyl-Histone H2B (Lys20) mouse mAb  (1:2000).  


Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine crotonylation is highly conserved in eukaryotic cellsfrom worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H2B at Lys20 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.  


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw cycles. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.

 

 **Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals


1. Tan MLuo HLee S, et al. Cell. 2011, 146(6):1016-1028.

ELISA, WB; Not tested in other applications 

*WB=Western blotting



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