修饰抗体 Products
组蛋白赖氨酸巴豆酰化 (crotonylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-crotonyl-Histone H3 (Lys14) mouse mAb

Cat#:PTM-537Ab Type:M

Ab Size:100 μlApplications:WB IP

Species:H M RPrice:¥ 3780.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by crotonylated Histone H3 at Lys14 peptide affinity chromatography. It specifically recognizes Histone H3 with crotonylation at Lys14.  


Source/Purification

This product is produced by immunizing mice with a synthetic crotonyl peptide corresponding to residues surrounding Lys14 of human histone H3. Antibodies are purified by protein G-conjugated agarose followed by crotonylated histone H3 (Lys14) peptide affinity chromatography.  


Specificity

H3

Recommended Applications

ELISA, WB, IP; Not tested in other applications  

*WB=Western blotting;  IP=Immunoprecipitation

Recommended antibody dilution: WB: 1:2000  


Recommended antibody dilution

WB: 1:2000 IP: 1:25~1:500

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

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Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine crotonylation is highly conserved in eukaryotic cellsfrom worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H3 at Lys14 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.  


Storage & Stability

 Store product at -20oC. Avoid repeated freeze/thaw cycles. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.  

 

 **Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.  


1. Zhang K, Chen Y, Zhang Z, et al. J Proteome Res. 2009, 8(2):900-906  

2. Cheng Z, Tang Y, Chen Y, et al. Mol Cell Proteomics. 2009, 8(1):45-52.  

3. Chen Y, Sprung R, Tang Y, et al. Mol Cell Proteomics. 2007, 6(5):812-819.  


ELISA, WB, IP; Not tested in other applications  

*WB=Western blotting;  IP=Immunoprecipitation

Recommended antibody dilution: WB: 1:2000  


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