修饰抗体 Products
组蛋白赖氨酸巴豆酰化 (crotonylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-crotonyl-Histone H3 (Lys18) rabbit pAb

Cat#:PTM-517Ab Type:P

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by crotonylated histone H3 (Lys18) peptide affinity chromatography. It specifically recognizes histone H3 with crotonylation at Lys18.  


Source/Purification

This product is produced by immunizing rabbits with a synthetic crotonyl peptide corresponding to residues surrounding Lys18 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by crotonylated histone H3 (Lys18) peptide affinity chromatography.  


Specificity

H3

Recommended Applications

ELISA, WB, IP; Not tested in other applications

*WB=Western blotting; IP= Immunoprecipation


Recommended antibody dilution

WB: 1:2000 IP: 1:25

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

 Anti-crotonyl-Histone H3 (Lys18) rabbit pAb  

A. Dot  blotting analysis on indicated amount of crotonylated H3 peptide at Lys18 (lane  1), butyrylated H3 peptide at Lys18 (lane 2), propionylated H3 peptide at Lys18  (lane 3) and unmodified H3 peptide at Lys18  (lane 4) using crotonyl-histone H3 (Lys18) rabbit pAb.


B. Western blotting analysis on 30 μg of crude proteins  from HeLa whole cell lysates with (right) or without (left) sodium crotonylate  treatment (30 mM, 4 hours) using crotonylate-histone H3 (Lys18) rabbit pAb (1:1000).  

  

Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine crotonylation is highly conserved in eukaryotic cellsfrom worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H3 at Lys18 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.  


Storage & Stability

Store product at -20 oC. Avoid repeated freeze/thaw cycles. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.

 

**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


1. Tan MLuo HLee S, et al. Cell. 2011, 146(6):1016-1028.

ELISA, WB, IP; Not tested in other applications

*WB=Western blotting; IP= Immunoprecipation


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