修饰抗体 Products
组蛋白赖氨酸巴豆酰化 (crotonylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-crotonyl-Histone H3 (Lys36) rabbit pAb

Cat#:PTM-536Ab Type:P

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description
Source/Purification

This product is produced by immunizing rabbits with a synthetic crotonyl peptide corresponding to residues surrounding Lys36 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by crotonylated histone H3 (Lys36) peptide affinity chromatography.  


Specificity

H2B

Recommended Applications

ELISA, Dot blot, Western blot.  

Recommended antibody dilution: WB: 1:2000  


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

图片11.png

A.Dot blotting analysis on indicated amount of peptides using histone H3K36cr rabbit pAb. The list of peptides is included in the table on the right.  

 

B. Western blotting analysis on 30 μg of crude proteins from MCF-7, COS-7, BRL, N2a whole cell lysates, and 200 ng recombinant histone H3 protein using crotonyl-Histone H3 (Lys36) rabbit pAb (1:2000).  

 

Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine crotonylation is highly conserved in eukaryotic cellsfrom worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H3 at Lys36 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.  


Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.

  


1. Tan M, Luo H, Lee S, et al. Cell. 2011, 146(6):1016-1028.

2. Andres, O.R. et al (2016) Biologists Ltd 10.1242/dmm.024455.  


ELISA, Dot blot, Western blot.  

Recommended antibody dilution: WB: 1:2000  


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