修饰抗体 Products
组蛋白赖氨酸2-羟基异丁基酰化(2-hydroxyisobutyrylation)修饰抗体
杭州景杰生物科技有限公司
Anti-2ohibutyryl-Histone H2B (Lys12) mouse mAb

Cat#:PTM-871Ab Type:M

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description
The mouse-derived antibody is purified with protein G-conjugated agarose followed by 2-hydroxyisobutyryl (2ohibutyryl) histone H2B (Lys12) peptide affinity chromatography. It specifically recognizes histone H2B with 2ohibutyrylation at Lys12.   
Source/Purification
This product is produced by immunizing mice with a synthetic 2ohibutyryl peptide corresponding to residues surrounding Lys12 of human histone H2B. Antibodies are purified by protein G-conjugated agarose followed by 2ohibutyrylated histone H2B (Lys12) peptide affinity chromatography.

Specificity

H2B

Recommended Applications
ELISA, WB; Not tested in other applications    
*WB=Western blotting; 

Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. 

Anti-2ohibutyryl-Histone H2B (Lys12) mouse mAb

Anti-2ohibutyryl-Histone H2B (Lys12) mouse mAb

A. Dot blotting analysis on indicated amount of 2ohibutyrylated H2B peptide at Lys12 (lane 1), acetylated H2B peptide at Lys11 (lane 2), butyrylated H2B peptide at Lys11 (lane 3), crotonyylated H2B peptide at Lys5 (lane 4), 3ohbutyrylated H2B peptide at Lys11 (lane 5), acetylated H2B peptide at Lys12 (lane 6), butyrylated H2B peptide at Lys12 (lane 7), crotonyylated H2B peptide at Lys12 (lane 8), 3ohbutyrylated H2B peptide at Lys12 (lane 9), mono-methylated H2B peptide at Lys12 (lane 10), acetylated H2B peptide at Lys15 (lane 11), butyrylated H2B peptide at Lys15 (lane 12), crotonyylated H2B peptide at Lys15 (lane 13), 3ohbutyrylated H2B peptide at Lys15 (lane 14), mono-methylated H2B peptide at Lys15 (lane 15), and unmodified H2B peptide at Lys12 (lane 16) using 2ohibutyryl-histone H2B (Lys12) mouse mAb. 


 B. Western blotting analysis on 30 μg of crude proteins from C2C12 whole cell lysates with (right) or without (left) treatment of sodium butyrate (30 mM, 4 hours) using 2ohibutyryl-histone H2B (Lys12) mouse mAb (1:2000).  
 C. Western blotting analysis on 30 μg of crude proteins from MCF-7 whole cell lysates with (right) or without (left) treatment of sodium butyrate (30 mM, 4 hours) and Trichostatin A (500ng, 4 hours) using 2ohibutyryl-histone H2B (Lys12) mouse mAb (1:2000).  
Scientific Description
Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, crotonylation, succinylation etc. 2-hydroxyisobutyrylation of lysine is a newly identified reversible modification controlling chromosome structureand gene transcription. The reversible lysine 2-hydroxyisobutyrylation has been well demonstrated in eukaryotichistones from worm to human. The unique structure and genomiclocalization of histone lysine 2-hydroxyisobutyrylation suggest that it is mechanisticallyand functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone2-hydroxyisobutyrylation marks either active promoters or potential enhancers.

Storage & Stability

The antibody is delivered in powder form. After dissolved with the antibody reconstitution buffer, please store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. 


**For research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



 1.Lunzhi D.,etal.(2014). NAT. CHEM. BIOL.  Lysine 2-hydroxyisobutyrylation is a widely distributed active histone mark. 

ELISA, WB; Not tested in other applications    
*WB=Western blotting; 


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