修饰抗体 Products
组蛋白赖氨酸2-羟基异丁基酰化(2-hydroxyisobutyrylation)修饰抗体
杭州景杰生物科技有限公司
Anti-2ohibutyryl-Histone H2B (Lys12) rabbit pAb

Cat#:PTM-831Ab Type:P

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3680.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by 2-hydroxyisobutyryl (2ohibutyryl) histone H2B (Lys12) peptide affinity chromatography. It specifically recognizes histone H2B with 2-ohibutyrylation at Lys12.



Source/Purification

This product is produced by immunizing rabbits with a synthetic 2ohibutyryl peptide corresponding to residues surrounding Lys12 of human histone H2B. Antibodies are purified by protein A-conjugated agarose followed by 2ohibutyrylated histone H2B (Lys12) peptide affinity chromatography.



Specificity

H2B

Recommended Applications

ELISA, WB; Not tested in other applications 

*WB=Western blotting


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

 Anti-2ohibutyryl-Histone H2B (Lys12) rabbit pAb 

A. Dot blotting analysis on indicated amount of 2ohibutyrylated H2B peptide at Lys12 (lane 1), 2ohibutyrylated H2B peptide at Lys5 (lane 2), crotonyylated H2B peptide at Lys11 (lane 3), 3ohbutyrylated H2B peptide at Lys11 (lane 4), acetylated H2B peptide at Lys12 (lane 5), crotonyylated H2B peptide at Lys12 (lane 6), 3ohbutyrylated H2B peptide at Lys12 (lane 7), 2ohibutyrylated H2B peptide at Lys20 (lane 8), and unmodified H2B peptide at Lys12 (lane 9) using 2ohibutyryl-histone H2B (Lys12) rabbit pAb.  

 

B. Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates treatment with (lane 2,3,4) or without (lane 1) sodium butyrate (30 mM, 4hr) using 2ohibutyryl-histone H2B (Lys12) rabbit pAb (1:2000) with no peptide blocking (lane 1, 4) or pre-adsorbed with 1.5 μM of the 2ohibutyrylated H2B at Lys12 peptides (lane 2) or unmodified H2B peptides at Lys 12 (lane3) .  


Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. 2-hydroxyisobutyrylation of lysine is a newly identified reversible modification controlling chromosome structureand gene transcription. The reversible lysine 2-hydroxyisobutyrylation has been well demonstrated in eukaryotichistones from worm to human. The unique structure and genomiclocalization of histone lysine 2-hydroxyisobutyrylation suggest that it is mechanisticallyand functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone2-hydroxyisobutyrylation marks either active promoters or potential enhancers.



Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



1. Lunzhi D.,etal.(2014). NAT. CHEM. BIOL.  Lysine 2-hydroxyisobutyrylation is a widely distributed active histone mark.

ELISA, WB; Not tested in other applications 

*WB=Western blotting


推荐产品 PRODUCTS
Copyright ©2018-2020 杭州景杰生物科技有限公司
犀牛云提供云计算服务
X
合作
交流
1

QQ设置

3

SKYPE 设置

4

阿里旺旺设置

等待加载动态数据...

等待加载动态数据...

5

电话号码管理

  • 400-100-1145
6

二维码管理

等待加载动态数据...

等待加载动态数据...

展开
合作交流
填写信息,我们的专属学术顾问将为您解答疑问!