修饰抗体 Products
组蛋白赖氨酸2-羟基异丁基酰化(2-hydroxyisobutyrylation)修饰抗体
杭州景杰生物科技有限公司
Anti-2ohibutyryl-Histone H4 (Lys5 8 12) rabbit pAb

Cat#:PTM-850Ab Type:P

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3680.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by 2-hydroxyisobutyryl(2ohibutyryl) histone H4 (Lys5/8/12) peptide affinity chromatography. It specifically recognizes histone H4 with 2ohibutyrylation at Lys5/8/12.

 

Source/Purification

This product is produced by immunizing rabbits with a synthetic 2ohibutyryl peptide corresponding to residues surrounding Lys5/8/12 of human histone H4. Antibodies are purified by protein A-conjugated agarose followed by 2ohibutyrylated histone H4 (Lys5/8/12) peptide affinity chromatography.


Specificity

H4

Recommended Applications

ELISA,WB; Not tested in other applications 

*WB=Western blotting


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user.  

 

Anti-2ohibutyryl-Histone H4 (Lys5 8 12) rabbit pAb

A. Dot blotting analysis on indicated amount of 2ohibutyrylated H4 peptide at Lys5/8/12 (lane 1), 2ohibutyrylated H4 peptide at Lys5 (lane 2), 2ohibutyrylated H4 peptide at Lys8 (lane 3), 2ohibutyrylated H4 peptide at Lys12 (lane 4), butyrylated H4 peptide at Lys5 (lane 5), 3ohbutyrylated H4 peptide at Lys5 (lane 6), crotonylated H4 peptide at Lys5 (lane 7), butyrylated H4 peptide at Lys8 (lane 8), 3ohbutyrylated H4 peptide at Lys8 (lane 9), 3ohbutyrylated H4 peptide at Lys12 (lane 10), 2ohibutyrylated H4 peptide at Lys16 (lane 11) and unmodified H4 peptide at Lys5/8/12 (lane 12) using 2ohibutyryl-histone H4 (Lys5/8/12) rabbit pAb.  


B.  Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (lane 2) or without (lane 1) sodium butyrate (30 mM, 4 hours) or sodium pyruvate (100 mM, 72hr) using 2ohibutyryl-histone H4 (Lys5/ 8/12) rabbit pAb(1:2000).  


Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. 2-hydroxyisobutyrylation of lysine is a newly identified reversible modification controlling chromosome structureand gene transcription. The reversible lysine 2-hydroxyisobutyrylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomiclocalization of histone lysine 2-hydroxyisobutyrylation suggest that it is mechanisticallyand functionally different from histonelysine acetylation. Specifically, in both humansomatic and mouse male germ cell genomes, histone2-hydroxyisobutyrylation marks either active promoters or potential enhancers.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



1. Lunzhi D.,etal.(2014). NAT. CHEM. BIOL.  Lysine 2-hydroxyisobutyrylation is a widely distributed acti-ve histone mark

ELISA,WB; Not tested in other applications 

*WB=Western blotting


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