修饰抗体 Products
组蛋白赖氨酸2-羟基异丁基酰化(2-hydroxyisobutyrylation)修饰抗体
杭州景杰生物科技有限公司
2-hydroxyisobutyryl-Histone H3 (Lys18) mouse mAb

Cat#:PTM-882Ab Type:M

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3680.00


Product Description
Source/Purification

This product is produced by immunizing mice with a synthetic 2-ohibutyryl peptide corresponding to residues surrounding Lys18 of human histone H3. Antibodies are purified by protein G-conjugated agarose followed by 2ohibutyrylated histone H3 (Lys18) peptide affinity chromatography.


Specificity

H3

Recommended Applications

ELISA, Dot blot, Western blot.  

Recommended antibody dilution: WB: 1:2000.


Recommended antibody dilution

1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

捕获16.PNG

捕获17.PNG

A. Dot blotting analysis on indicated amount of peptides using histone H3K18hib mouse mAb. The list of peptides is included in the table on the right.


B. Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates treatment with (lane 2,3,4) or without (lane 1) sodium butyrate (30 mM, 4hr) using 2ohibutyryl-histone H3 (Lys18) mouse mAb (1:2000) with no peptide blocking (lane 1, 4) or pre-adsorbed with 1.5 μM of the 2ohibutyrylated H3 at Lys18 peptides (lane 2) or unmodified H3 peptides at Lys 18 (lane 3).


Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. 2-hydroxyisobutyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine 2-hydroxyisobutyrylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine 2-hydroxyisobutyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone 2-hydroxyisobutyrylation marks either active promoters or potential enhancers.


Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.



1. Dai, L., C. Peng, et al. (2014). "Lysine 2-hydroxyisobutyrylation is a widely distributed active histone mark." Nat Chem Biol 10(5): 365-370.


ELISA, Dot blot, Western blot.  

Recommended antibody dilution: WB: 1:2000.


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