修饰抗体 Products
组蛋白赖氨酸琥珀酰化 (succinylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-succinyl-Histone H3 (Lys23) mouse mAb

Cat#:PTM-422Ab Type:M

Ab Size:100 μlApplications:WB, IHC

Species:H M RPrice:¥ 3780.00


Product Description

The antibody is purified with protein G-conjugated agarose followed by succinylated histone H3 (Lys23) peptide affinity chromatography. It specifically recognizes histone H3 with lysine succinylation at Lys14.


Source/Purification

This product is produced by immunizing mice with a synthetic succinyl peptide corresponding to residues surrounding Lys23 of human histone H3. Antibodies are purified by protein G-conjugated agarose followed by succinylated histone H3 (Lys23) peptide affinity chromatography.


Specificity

H3

Recommended Applications

ELISA, WB, IHC; Not tested in other applications

*WB=Western blotting    IHC=Immunohistochemistry


Recommended antibody dilution

WB: 1:2000    IHC: 1:200

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  



Anti-succinyl-Histone H3 (Lys23) mouse mAb

Figure ASuccinyl-Histone H3 (Lys23) mouse mAb specificity was determined by peptide ELISA. The graph depicts the binding of the antibody to pre-coated succinyl-histone H3 (Lys23) peptide in the presence of various competitor peptides. As shown, only the succinyl-histone H3 (Lys23) peptide competed away binding of the antibody.


                     

Anti-succinyl-Histone H3 (Lys23) mouse mAb

Figure B: Western blotting analysis on 30 μg of crude proteins from human HeLa whole cell lysates with (Lane”+”) or without (Lane “-“) treatment of sodium butyrate (30 mM, 4 hours) or mouse N2a whole cell lysates using succinyl-histone H3 (Lys23) mouse mAb (1: 2000).



Anti-succinyl-Histone H3 (Lys23) mouse mAb

Figure C. Immunohistochemistry analysis of paraffin-embedded human neuroblastoma tissue, labeling H3K23su with PTM-422 at 1/200 dilution. 

Nuclear staining on tumor cells of human neuroblastoma was observed. Counter stained with hematoxylin.



Scientific Description

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Lysine succinylation is a recently identified novel Protein Post-translational Modification (PTM). Lysine succinylation is evolutionarily conserved and abundantly present in proteins in both prokaryotic and eukaryotic cells, suggesting its roles in regulating cellular physiology. This antibody will facilitate studies of lysine succinylation biology by identifying its substrates and studying its function.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



1. Zhang Z, Tan M, Xie Z, et al. (2011) Nat Chem Biol. 201, 7(1):58-63.

2. Peng C, Liu Z, Xie Z, et al. (2011) Molecular and Cellular Proteomics. 10(12):M111.012658.

3. Xie Z, Dai J, Dai L, et al. (2012) Molecular and Cellular Proteomics. 11(5):100-107


ELISA, WB, IHC; Not tested in other applications

*WB=Western blotting    IHC=Immunohistochemistry


推荐产品 PRODUCTS
Copyright ©2018-2020 杭州景杰生物科技有限公司
犀牛云提供云计算服务
X
合作
交流
1

QQ设置

3

SKYPE 设置

4

阿里旺旺设置

等待加载动态数据...

等待加载动态数据...

5

电话号码管理

  • 400-100-1145
6

二维码管理

等待加载动态数据...

等待加载动态数据...

展开
合作交流
填写信息,我们的专属学术顾问将为您解答疑问!