修饰抗体 Products
组蛋白赖氨酸琥珀酰化 (succinylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-succinyl-Histone H3 (Lys14) mouse mAb

Cat#:PTM-421Ab Type:M

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description

The antibody is purified with protein G-conjugated agarose followed by succinylated histone H3 (Lys14) peptide affinity chromatography. It specifically recognizes histone H3 with lysine succinylation at Lys14.


Source/Purification

This product is produced by immunizing mice with a synthetic succinyl peptide corresponding to residues surrounding Lys14 of human histone H3. Antibodies are purified by protein G-conjugated agarose followed by succinylated histone H3 (Lys14) peptide affinity chromatography.


Specificity

H3

Recommended Applications

ELISA, WB; Not tested in other applications

*WB=Western blotting


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  


Anti-succinyl-Histone H3 (Lys14) mouse mAbNO.PeptideNO.Peptide
Lane 1H3K14suLane 2H3K14ac
Lane 3H3K14prLane 5H3K14bu
Lane 4H3K14crLane 6H3K14un

Figure A:Dot blotting analysis on indicated amount of peptide using histone H3K14su mouse mAb. The list of peptides is included in the above table.


                        Anti-succinyl-Histone H3 (Lys14) mouse mAb

Figure B: Western blotting analysis on 30 μg of crude proteins from MCF-7 whole cell lysates with (right) or without (left) treatment of sodium butyrate (30 mM, 4 hours) and Trichostatin A (500ng, 4 hours) using succinyl-histone H3 (Lys14) mouse mAb (1: 2000). 



Scientific Description

Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Lysine succinylation is a recently identified novel Protein Post-translational Modification (PTM). Lysine succinylation is evolutionarily conserved and abundantly present in proteins in both prokaryotic and eukaryotic cells, suggesting its roles in regulating cellular physiology. This antibody will facilitate studies of lysine succinylation biology by identifying its substrates and studying its function.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



1. Zhang Z, Tan M, Xie Z, et al. (2011) Nat Chem Biol. 201, 7(1):58-63.

2. Peng C, Liu Z, Xie Z, et al. (2011) Molecular and Cellular Proteomics. 10(12):M111.012658.

3. Xie Z, Dai J, Dai L, et al. (2012) Molecular and Cellular Proteomics. 11(5):100-107


ELISA, WB; Not tested in other applications

*WB=Western blotting


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