修饰抗体 Products
组蛋白H2B抗体
杭州景杰生物科技有限公司
Anti-histone H2B mouse mAb

Cat#:

PTM-1006

Ab Type:

M

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

1380.00

Price: (20 μl)

414.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by affinity chromatography on immunogen peptide. It specifically recognizes endogenous histone H2B protein, not cross-react with other histones.  


Source/Purification

This product is produced by immunizing mice with a synthetic peptide corresponding to N terminus of human histone H2B. Antibodies are purified by peptide affinity chromatography.  


Specificity

H2B

Recommended Applications

ELISA,WB; Not tested in other applications  

*WB=Western blotting  


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

Anti-histone H2B mouse mAb

A.Western blotting analysis on 30 μg of crude proteins from human breast MCF-7, rat liver BRL and mouse neuroblastoma N2a whole cell lysates using histone H2B mouse mAb (1:2000).  


B.Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (“+”) or without (“-“) treatment of nocodazole (100 ng/ml, 18hr) and calyculin A (100 nM, 1hr) using histone H2B mouse mAb (1:2000).The membrane was re-probed by anti-phospho-histone H2A.x (Ser139) mouse mAb.  


Scientific Description

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries. Featuring a main globular domain and a long N-terminal tail, H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H4 is the most extensively modified of the five histones. It is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.  


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.  

 

**For research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.  


Zhou X,et al,Systematic Analysis of the Lysine Acetylome in Candida albicans. J Proteome Res. 2016 Aug 5;15(8):2525-36. doi: 10.1021/acs.jproteome.6b00052. Epub 2016 Jun 28.

ELISA,WB; Not tested in other applications  

*WB=Western blotting


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