修饰抗体 Products
组蛋白H2B抗体
杭州景杰生物科技有限公司
Anti-histone H2B rabbit pAb (CT)

Cat#:

PTM-1007

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

1380.00

Price: (20 μl)

414.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by affinity chromatography on immunogen peptide. It specifically recognizes endogenous histone H2A protein and doesnot cross-react with other histones.   


Source/Purification

This product is produced by immunizing rabbits with a synthetic peptide corresponding to C terminus of human histone H2A. Antibodies are purified by peptide affinity chromatography.  


Specificity

H2B

Recommended Applications

ELISA, Dot blot, Western blot. 

Recommended antibody dilution: WB: 1:2000


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

Anti-histone H2B rabbit pAb (CT)   

A. Western blotting analysis on 30 μg of crude proteins from HeLa, COS-7, BRL whole cell lysates, and Liver, Kidney tissue lysate using histone H2A rabbit pAb (1:2000).   

 

B. Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (right) or without (left) treatment of sodium butyrate (30 mM, 4hr) using histone H2A rabbit pAb (1:2000).The membrane was re-probed by anti-acetyl-histone H2A (Lys5) rabbit pAb (1:2000).  

 

Scientific Description

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries. Histone H2B plays an important role in the biology of the nucleus where it is involved in the packaging and maintaining of chromosomes, and repair of DNA. Histone H2B helps regulate chromatin structure and function through post-translational modifications and specialized histone variants. It is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.


Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.


ELISA, Dot blot, Western blot. 

Recommended antibody dilution: WB: 1:2000


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