修饰抗体 Products
组蛋白H4抗体
杭州景杰生物科技有限公司
Crotonyl-Histone H4 (Ser1) rabbit pAb

Cat#:PTM-541Ab Type:P

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description
Source/Purification

This product is produced by immunizing rabbits with a synthetic crotonyl peptide corresponding to residues surrounding Ser1 of human histone H4. Antibodies are purified by protein A-conjugated agarose followed by crotonylated histone H4 (Ser1) peptide affinity chromatography.


Specificity

H4

Recommended Applications

ELISA, Dot blot, Western blot.

Recommended antibody dilution: WB: 1:2000


Recommended antibody dilution

1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.

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A. Dot blotting analysis on indicated amount of peptides using histone H4S1cr rabbit pAb. The list of peptides is included in the table on the right.


B.Western blotting analysis on 20 μg histone proteins extracted from JK cell or mouse spleen tissure using crotonyl-histone H4 (Ser1) rabbit pAb (1: 2000).


Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. Lysine crotonylation is highly conserved in eukaryotic cells from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and rabbit male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H4 at Ser1 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.


Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.


1. Tan M, Luo H, Lee S, et al. Cell. 2011, 146(6):1016-1028.

ELISA, Dot blot, Western blot.

Recommended antibody dilution: WB: 1:2000


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