修饰抗体 Products
组蛋白H4抗体
杭州景杰生物科技有限公司
Histone H4 rabbit pAb (NT)

Cat#:

PTM-1003

Ab Type:

P

Applications:

WB, ELISA, ICC, IHC

Species:

Human, Mouse, Rat

Price: (100 μl)

1380.00

Price: (20 μl)

414.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by histone H4 peptide affinity chromatography. It specifically recognizes histone H4 protein.


Source/Purification

This product is produced by immunizing rabbits with a synthetic peptide corresponding to N terminus of human histone H4. Antibodies are purified by peptide affinity chromatography.


Specificity

H4

Recommended Applications

ELISA, WB, ICC, IHC; Not tested in other applications

*WB=Western blotting; IHC= Immunohistochemistry; ICC=Immunocytochemistry


Recommended antibody dilution

WB: 1:2000    IHC: 1:200    ICC: 1:200


Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  


Histone H4 rabbit pAb

Western blotting  analysis on 30 μg of crude proteins from human HeLa, mouse C2C12 and monkey COS-7 cell(Figure A)or 30 μg of crude proteins from HeLa whole cell lysates with (right) or without (left) sodium butyrate treatment (30 mM, 4 hours) (Figure B) using histone H4 rabbit pAb (1:2000).The membrane was re-probed by acetyl-histone H4 (Lys12) rabbit pAb.

Histone H4 rabbit pAb (NT)

Figure C. Immunohistochemical analysis of paraffin-embedded human neuroblastoma tissue, labeling H4 with PTM-1003 at 1/200 dilution. Nuclear staining on tumor cells of human neuroblastoma was observed. Counter stained with hematoxylin.

Histone H4 rabbit pAb (NT)

Figure D. Immunocytochemistry analysis of Hela (Human cervix adenocarcinoma cell line), labeling H4 with PTM-1003 at 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Nuclei were stained with DAPI (blue).



Scientific Description

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries. Featuring a main globular domain and a long N-terminal tail, H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H4 is the most extensively modified of the five histones. It is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.


Storage & Stability

Store product at -20℃. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**This product is intended for research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



Recommended Applications

ELISA, WB, ICC, IHC; Not tested in other applications

*WB=Western blotting; IHC= Immunohistochemistry; ICC=Immunocytochemistry


Recommended antibody dilution

WB: 1:2000    IHC: 1:200    ICC: 1:200


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