修饰抗体 Products
组蛋白H4抗体
杭州景杰生物科技有限公司
Anti-histone H4 mouse mAb

Cat#:

PTM-1009

Ab Type:

M

Applications:

WB, Dot, ELISA, IHC

Species:

Human, Mouse, Rat, Arabidopsis thaliana, Rice

Price: (100 μl)

1380.00

Price: (20 μl)

414.00


Product Description
Source/Purification

This product is produced by immunizing Balb/c mice with a synthetic peptide corresponding to N terminus of human histone H4. Antibodies are purified by protein G-conjugated agarose

 

Specificity

H4

Recommended Applications

ELISA, Dot blot, WB, IHC. Not tested in other applications  

*WB=Western blottingIHC=Immunohistochemistry


Recommended antibody dilution

WB: 1:2000    IHC: 1:500

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

捕获23.JPG


    

Anti-histone H4 mouse mAb

D.

       Anti-histone H4 mouse mAb


Western blotting analysis on 30 μg of crude proteins from MCF-7, BRL, N2a, COS-7  whole cell lysates (Figure A) and 30 μg of crude proteins from HeLa whole cell lysates with (right) or without (left) treatment of Trichostatin A (500ng, 4 hours) (Figure B) using histone H4 mouse mAb(1:2000). The membrane was re-probed by anti- acetyl-histone H4 (Lys5) mouse mAb.

Western blotting analysis on 30 μg of crude proteins from Rice, Arabidopsis thaliana histone lysates(Figure C) using histone H4 mouse mAb(1:400).


Immunohistochemical analysis of paraffin-embedded human neuroblastoma tissue, labeling H4 with PTM-1009 at 1/500 dilution.

Nuclear staining on tumor cells of human neuroblastoma was observed. Counter stained with hematoxylin.



Scientific Description

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries. Featuring a main globular domain and a long N-terminal tail, H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H4 is the most extensively modified of the five histones. It is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. 


Storage & Stability

The antibody is delivered in powder form. Before use, please read carefully the Reconstitution and Storage Instructions. After dissolved with the antibody reconstitution buffer, please store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. 



ELISA, Dot blot, WB, IHC. Not tested in other applications  

*WB=Western blottingIHC=ImmunohistochemistryICC=Immunocytochemistry

WB: 1:2000    IHC: 1:500


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