修饰抗体 Products
组蛋白赖氨酸β-羟基丁酰化(β-Hydroxybutyrylation)修饰抗体
杭州景杰生物科技有限公司
β-hydroxybutyryl-Histone H3 (Lys4) rabbit pAb

Cat#:PTM-1258Ab Type:P

Ab Size:100 μlApplications:WB IP

Species:H M RPrice:¥ 3680.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by β-hydroxybutyryl (3ohbutyryl) histone H3 (Lys4) peptide affinity chromatography. It specifically recognizes histone H3 with β-hydroxybutyrylation at Lys4.


Source/Purification

This product is produced by immunizing rabbits with a synthetic 3ohbutyryl peptide corresponding to residues surrounding Lys4 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by 3ohbutyrylated histone H3 (Lys4) peptide affinity chromatography.  


Specificity

H3

Recommended Applications

ELISA,WB, IP; Not tested in other applications   

*WB=Western blotting IP=Immunoprecipitation


Recommended antibody dilution

WB: 1:2000 IP: 1:25~1:50

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

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Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. β-hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia.  


Storage & Stability

Store product at -20℃. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.


Zhongyu X, Di Z., etal.(2016). Mol. cell. 62, 194-206.  Metabolic regulation of gene expression by histone lysine

 


ELISA,WB, IP; Not tested in other applications   

*WB=Western blotting IP=Immunoprecipitation


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