修饰抗体 Products
组蛋白赖氨酸β-羟基丁酰化(β-Hydroxybutyrylation)修饰抗体
杭州景杰生物科技有限公司
β-hydroxybutyryl-Histone H3 (Lys4) mouse mAb

Cat#:PTM-1296Ab Type:M

Ab Size:100 μlApplications:WB IP

Species:H M RPrice:¥ 3680.00


Product Description
Source/Purification

This product is produced by immunizing mice with a synthetic 3-ohbutyryl peptide corresponding to residues surrounding Lys4 of human histone H3. Antibodies are purified by protein G-conjugated agarose followed by 3ohbutyryl histone H3 (Lys4) peptide affinity chromatography.


Specificity

H3

Recommended Applications

ELISA,WB, IP; Not tested in other applications   

*WB=Western blotting IP=Immunoprecipitation


Recommended antibody dilution

WB: 1:2000 IP: 1:25~1:200

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

blob.png



blob.png

Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. β-hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia.


Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.


Zhongyu X, Di Z., etal.(2016). Mol. cell. 62, 194-206.  Metabolic regulation of gene expression by histone lysine β-hydroxybutyrylation.

ELISA,WB, IP; Not tested in other applications   

*WB=Western blotting IP=Immunoprecipitation


推荐产品 PRODUCTS
Copyright ©2018-2020 杭州景杰生物科技有限公司
犀牛云提供云计算服务
X
合作
交流
1

QQ设置

3

SKYPE 设置

4

阿里旺旺设置

等待加载动态数据...

等待加载动态数据...

5

电话号码管理

  • 400-100-1145
6

二维码管理

等待加载动态数据...

等待加载动态数据...

展开
合作交流
填写信息,我们的专属学术顾问将为您解答疑问!