修饰抗体 Products
组蛋白赖氨酸β-羟基丁酰化(β-Hydroxybutyrylation)修饰抗体
杭州景杰生物科技有限公司
β-hydroxybutyryl-Histone H2B (Lys23) rabbit pAb

Cat#:PTM-1236Ab Type:P

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3680.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by β-hydroxybutyryl (3ohbutyryl) histone H2B (Lys23) peptide affinity chromatography. It specifically recognizes histone H2B with β-hydroxybutyrylation at Lys23.


Source/Purification

This product is produced by immunizing rabbits with a synthetic 3ohbutyryl peptide corresponding to residues surrounding Lys23 of human histone H2B. Antibodies are purified by protein A-conjugated agarose followed by 3ohbutyryl histone H2B (Lys23) peptide affinity chromatography.  


Specificity

H2B

Recommended Applications

ELISA, WB. Not tested in other applications  

*WB=Western blotting  


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

β-hydroxybutyryl-Histone H2B (Lys23) rabbit pAb 

A.Dot blotting analysis on indicated amount of 3ohbutyrylated H2B peptide at Lys23 (lane 1), crotonylated H2B peptide at Lys16 (lane 2), 3ohbutyrylated H2B peptide at Lys16 (lane 3), butyrylated H2B peptide at Lys20 (lane 4), crotonylated H2B peptide at Lys20 (lane 5), 2ohibutyrylated H2B peptide at Lys20 (lane 6), 3ohbutyrylated H2B peptide at Lys20 (lane 7), crotonylated H2B peptide at Lys23 (lane 8), 2ohibutyrylated H2B peptide at Lys23 (lane 9), 3ohbutyrylated H2B peptide at Lys24 (lane 10) and unmodified H2B peptide at Lys23 (lane 11) using β-hydroxybutyryl-histone H2B (Lys23) rabbit pAb.  


B.Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (lane 2) or without (lane 1) sodium 3-hydroxybutyrate (50 mM, 72hr) using β-hydroxybutyryl -histone H2B (Lys23) rabbit pAb(1:2000).  


Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. β-hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia.  


Storage & Stability

Store product at -20 ℃. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from the date of receipt.


Zhongyu X, Di Z., etal.(2016). Mol. cell. 62, 194-206.  Metabolic regulation of gene expression by histone lysine

ELISA, WB. Not tested in other applications  

*WB=Western blotting  


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