修饰抗体 Products
组蛋白赖氨酸β-羟基丁酰化(β-Hydroxybutyrylation)修饰抗体
杭州景杰生物科技有限公司
β-hydroxybutyryl-Histone H3 (Lys18) mouse mAb

Cat#:PTM-1292Ab Type:M

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3680.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by β-hydroxybutyryl (3-ohbutyryl) histone H3 (Lys18) peptide affinity chromatography. It specifically recognizes histone H3 with β-hydroxybutyrylation at Lys18.


Source/Purification

This product is produced by immunizing mice with a synthetic 3-ohbutyryl peptide corresponding to residues surrounding Lys18 of human histone H3. Antibodies are purified by protein G-conjugated agarose followed by 3ohbutyryl histone H3 (Lys18) peptide affinity chromatography.  


Specificity

H3

Recommended Applications

ELISA,WB; Not tested in other applications  

*WB=Western blotting;  


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

β-hydroxybutyryl-Histone H3 (Lys18) mouse mAb

A.Dot blotting analysis on indicated amount of 3ohbutyrylated H3 peptide at Lys18 (lane 1), 2ohibutyrylated H3 peptide at Lys9 (lane 2), 3ohbutyrylated H3 peptide at Lys9 (lane 3), 2ohibutyrylated H3 peptide at Lys14 (lane 4), 3ohbutyrylated H3 peptide at Lys14 (lane 5), mono-methylated H3 peptide at Lys14 (lane 6), di-methylated H3 peptide at Lys14 (lane 7), acetylated H3 peptide at Lys18 (lane 8), propionylated H3 peptide at Lys18 (lane 9), butyrylated H3 peptide at Lys18 (lane 10), crotonylated H3 peptide at Lys18 (lane 11), mono-methylated H3 peptide at Lys18 (lane 12), tri-methylated H3 peptide at Lys18 (lane 13), 2ohibutyrylated H3 peptide at Lys23 (lane 14), 3ohbutyrylated H3 peptide at Lys23 (lane 15), mono-methylated H3 peptide at Lys23 (lane 16), tri-methylated H3 peptide at Lys23 (lane 17), and unmodified H3 peptide at Lys18 (lane 18) using β-hydroxybutyryl-histone H3 (Lys18) mouse mAb.  

 

B.Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (lane 2) or without (lane 1) sodium 3-hydroxybutyrate (50 mM, 72hr) using β-hydroxybutyryl-histone H3 (Lys18) mouse mAb (1:2000).  

 

Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. β-hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia.  


Storage & Stability

Store product at -20 ℃. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.



Zhongyu X, Di Z., etal.(2016). Mol. cell. 62, 194-206.  Metabolic regulation of gene expression by histone lysine

ELISA,WB; Not tested in other applications  

*WB=Western blotting;  


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