修饰抗体 Products
组蛋白赖氨酸β-羟基丁酰化(β-Hydroxybutyrylation)修饰抗体
杭州景杰生物科技有限公司
β-hydroxybutyryl-Histone H3 (Lys23) rabbit pAb

Cat#:PTM-1300Ab Type:P

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3680.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by β-hydroxybutyryl(3-ohbutyryl) histone H3 (Lys23) peptide affinity chromatography. It specifically recognizes histone H3 with β-hydroxybutyrylation at Lys23.


Source/Purification

This product is produced by immunizing rabbits with a synthetic 3-ohbutyryl peptide corresponding to residues surrounding Lys23 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by 3ohbutyrylhistone H3 (Lys23) peptide affinity chromatography.  


Specificity

H3

Recommended Applications

 ELISA, WB. Not tested in other applications  

*WB=Western blotting  


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

 β-hydroxybutyryl-Histone H3 (Lys23) rabbit pAb

A. Dot blotting analysis on indicated amount of 3ohbutyrylated H3 peptide at Lys23 (lane 1), propionylated H3 peptide at Lys18 (lane 2), butyrylated H3 peptide at Lys18 (lane 3), crotonylated H3 peptide at Lys18 (lane 4), 2ohibutyrylated H3 peptide at Lys18 (lane 5), 3ohbutyrylated H3 peptide at Lys18 (lane 6), propionylated H3 peptide at Lys23 (lane 7), butyrylated H3 peptide at Lys23 (lane 8), crotonylated H3 peptide at Lys23 (lane 9), 2ohibutyrylated H3 peptide at Lys23 (lane 10), mono-methylated H3 peptide at Lys23 (lane 11), crotonylated H3 peptide at Lys27 (lane 12), 2ohibutyrylated H3 peptide at Lys27 (lane 13), 3ohbutyrylated H3 peptide at Lys27 (lane 14), and unmodified H3 peptideat Lys23 (lane 15) using β-hydroxybutyryl-histone H3 (Lys23) rabbit pAb.  

 

B.Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (lane 2) or without (lane 1) sodium 3-hydroxybutyrate (50 mM, 72hr) using β-hydroxybutyryl-histone H3 (Lys23) rabbit pAb (1:2000).  

 

Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. β-hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia.  


Storage & Stability

Store product at -20℃. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from the date of receipt.


Zhongyu X, Di Z., etal.(2016). Mol. cell. 62, 194-206.  Metabolic regulation of gene expression by histone lysine

ELISA, WB. Not tested in other applications  

*WB=Western blotting  


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