修饰抗体 Products
组蛋白赖氨酸β-羟基丁酰化(β-Hydroxybutyrylation)修饰抗体
杭州景杰生物科技有限公司
β-hydroxybutyryl-Histone H3 (Lys27) mouse mAb

Cat#:PTM-1293Ab Type:M

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3680.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by β-hydroxybutyryl (3-ohbutyryl) histone H3 (Lys27) peptide affinity chromatography. It specifically recognizes histone H3 with β-hydroxybutyrylation at Lys27.


Source/Purification

This product is produced by immunizing mice with a synthetic 3-ohbutyryl peptide corresponding to residues surrounding Lys27 of human histone H3. Antibodies are purified by protein G-conjugated agarose followed by 3ohbutyryl histone H3 (Lys27) peptide affinity chromatography.  


Specificity

H3

Recommended Applications

ELISA, WB. Not tested in other applications  

*WB=Western blotting  


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

β-hydroxybutyryl-Histone H3 (Lys27) mouse mAb

A. 3ohbutyryl-Histone H3 (Lys27) mouse mAb specificity was determined by peptide ELISA. The graph depicts the binding of the antibody to pre-coated 3ohbutyryl-histone H3(Lys27) peptide in the presence of increasing concentrations of various competitor peptides.As shown, only the β-hydroxybutyryl-histone H3 (Lys27) peptide competed away binding of the antibody.  


B. Western blotting analysis on 30 μg of crude proteins from mouse heart, mouse liver whole cell lysate and 200 ng recombinant histone H3 protein using β-hydroxybutyryl-Histone H3 (Lys27) mouse mAb (1:500).  


C. Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates using β-hydroxybutyryl -histone H3 (Lys27) rabbit pAb (1:2000) with no peptide blocking (lane 3) or pre-adsorbed with 1.5 μM of the 3ohbutyrylated H3 at Lys27 peptides (lane 1) or unmodified H2B peptides at Lys 27 (lane2) .  


Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. β-hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia.  


Storage & Stability

Store product at -20 ℃. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.



Zhongyu X, Di Z., etal.(2016). Mol. cell. 62, 194-206.  Metabolic regulation of gene expression by histone lysine



ELISA, WB. Not tested in other applications  

*WB=Western blotting  


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