修饰抗体 Products
组蛋白赖氨酸β-羟基丁酰化(β-Hydroxybutyrylation)修饰抗体
杭州景杰生物科技有限公司
β-hydroxybutyryl-Histone H2A (Lys5) mouse mAb

Cat#:PTM-1270Ab Type:P

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3680.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by β-hydroxybutyryl (3-ohbutyryl) histone H2A (Lys5) peptide affinity chromatography. It specifically recognizes histone H2A with β-hydroxybutyrylation at Lys5.


Source/Purification

This product is produced by immunizing mice with a synthetic 3-ohbutyryl peptide corresponding to residues surrounding Lys5 of human histone H2A. Antibodies are purified by protein G-conjugated agarose followed by 3ohbutyryl histone H2A (Lys5) peptide affinity chromatography.  


Specificity

H2A

Recommended Applications

ELISA, WB. Not tested in other applications  

*WB=Western blotting  


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

 β-hydroxybutyryl-Histone H2A (Lys5) mouse mAb
 
  

   

A.Dot blotting analysis on indicated amount of 3ohbutyrylated H2A peptide at Lys5 (lane 1), acetylated H2A peptide at Lys5 (lane 2), 2ohibutyrylated H2A peptide at Lys5 (lane 3), succinylated H2A peptide at Lys9 (lane 4), 2ohibutyrylated H2A peptide at Lys9 (lane 5), 3ohbutyrylated H2A peptide at Lys9 (lane 6) and unmodified H2A peptide at Lys5 (lane 7) using β-hydroxybutyryl-histone H2A (Lys5) mouse mAb.  


B.Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (lane 2) or without (lane 1) sodium 3-hydroxybutyrate (50 mM, 72hr) using β-hydroxybutyryl-histone H2A (Lys5) mouse mAb (1:2000).  

 

Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. β-hydroxybutyrylation of lysine (Kbhb) is a newly identified histone marker that enriched in active gene promoters. Histone Kbhb marks are dramatically induced in response to elevated β-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. Histone β-hydroxybutyrylation represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of β-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia.  


Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.



Zhongyu X, Di Z., etal.(2016). Mol. cell. 62, 194-206.  Metabolic regulation of gene expression by histone lysine

ELISA, WB. Not tested in other applications  

*WB=Western blotting  


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